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Chinese pancreas cancer cell line sipanc-187 establishment method

A technology of pancreatic cancer cells and establishment methods, which is applied in the field of establishment of Chinese pancreatic cancer cell line sipanc-187, can solve problems such as application limitations, and achieve good tumorigenic effects

Pending Publication Date: 2018-08-10
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

In addition, due to the lack of blood supply of pancreatic cancer itself [5] , and many tumor-associated fibroblasts (cancer associated fibroblasts, CAF) enrichment characteristics, the application of traditional primary tumor cell line establishment methods in pancreatic cancer is greatly limited

Method used

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  • Chinese pancreas cancer cell line sipanc-187 establishment method
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  • Chinese pancreas cancer cell line sipanc-187 establishment method

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Embodiment Construction

[0016] The present invention will be described in detail below in conjunction with accompanying drawing:

[0017] The method for building a system of the present invention:

[0018] 1. Source of specimen: The specimen was taken from a 74-year-old female patient in the Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine. Pathological diagnosis: pancreatic ductal adenocarcinoma, TNM stage: T 3 N 1 m 0 .

[0019] 2. Specimen collection: Aseptically collect fresh pancreatic cancer tumor samples, immediately immerse in 20ml of complete medium (RPMI1640 configuration, containing final concentration of 10% FBS (v / v%) and penicillin / streptomycin double antibody 100mg / ml each) , use 20ml PBS to wash the tissue repeatedly in a safety fume hood, wash and remove visible blood clots and necrotic tissue, take a tumor with a diameter of 1*1*1-1.5*1.5*1.5cm, and cut it into 1mm 3 size organization block.

[0020] 3....

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Abstract

The invention discloses a Chinese pancreas cancer cell line sipanc-187 establishment method. The method includes the following steps: 1. specimen acquisition; 2. tissue digestion; 3. PDX nude mouse tumor-bearing; and 4. tumor cell culture and passage. The beneficial effects of the invention are that a Chinese pancreas cancer cell line sipanc-187 established according to the invention is biologically characterized by pancreatic ductal adenocarcinoma, wherein the Chinese pancreas cancer cell line sipanc-187 has good tumorigenicity and certain medicine resistance. The Chinese pancreas cancer cellline sipanc-187 can be greatly applied to research of pancreas cancer tumors and a chemotherapy medicine-resistance mechanism, pedigree analysis of Chinese pancreas cancer related genes, and the like.

Description

technical field [0001] The invention relates to a method for establishing a primary tumor cell line of pancreatic cancer, mainly a method for establishing a Chinese pancreatic cancer cell line sipanc-187. Background technique [0002] Pancreatic ductal adenocarcinoma (Pancreatic ductal adenocarcinoma, PDAC) is the most common pancreatic tumor. Metastasis occurs in the early stage, but the symptoms are not obvious. When it is discovered, most of them are in the advanced stage, and the prognosis is extremely poor. The five-year survival rate after diagnosis is often less than 5%. Known as the "King of Cancer" [1-3] , ranking sixth in the death rate of male cancer patients in my country [4] . Most of the pancreatic cancer cell lines used in basic and clinical research in the past came from foreign ATCC cell banks, such as Panc-1, Bxpc-3, Miapaca-2, ASPC-1, etc., and the cell lines were derived from Caucasian pancreatic cancer patients , for pancreatic cancer research in my co...

Claims

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Application Information

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IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2509/00
Inventor 梁廷波白雪莉陈琦王健鑫魏涛王艺王建锋倪磊
Owner ZHEJIANG UNIV
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