Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus plantarum ccfm1019, its fermented food and its application in the preparation of medicine

A technology of CCFM1019 and Lactobacillus plantarum, which is applied in the field of microorganisms, can solve the problems of unsatisfactory adsorption effect, achieve good gastric acid resistance, bile salt resistance, reduce content, and reduce damage effects

Active Publication Date: 2020-05-12
JIANGNAN UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing literature reports, the highest adsorption rate of probiotics to DEHP in vitro is only 9.62%, and the adsorption effect is not ideal.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus plantarum ccfm1019, its fermented food and its application in the preparation of medicine
  • Lactobacillus plantarum ccfm1019, its fermented food and its application in the preparation of medicine
  • Lactobacillus plantarum ccfm1019, its fermented food and its application in the preparation of medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Lactobacillus plantarum CCFM1019 has good tolerance to simulated gastrointestinal fluid

[0069] The cryopreserved Lactobacillus plantarum CCFM1019 was streak-inoculated in the MRS solid medium, and cultured aerobically at 37°C for 24 hours, and then subcultured in the MRS medium for 2 to 3 times, then the culture medium of Lactobacillus plantarum CCFM1019 was taken, The bacteria were collected by centrifugation at 8000×g for 5 min, resuspended in (1:1) artificial simulated gastric juice of pH 2.5 (MRS medium containing 1% pepsin, pH=2.5), and then cultured aerobically at 37°C. Samples were taken at the beginning (0h), 1h, 2h and 3h, and MRS agar medium was used to pour culture for plate colony counting, and the number of viable bacteria was determined and the survival rate was calculated. The survival rate is the ratio of the number of viable bacteria in the culture medium to the number of viable bacteria at 0h, expressed in %.

[0070] Take the culture sol...

Embodiment 2

[0075] Example 2: Lactobacillus plantarum CCFM1019 has good adsorption capacity for DEHP and MEHP in an aqueous solution containing plasticizer DEHP or MEHP in vitro

[0076] Bacteria adsorption: After the experimental bacteria were purified and activated, they were inserted into the MRS liquid medium according to the inoculation amount of 1% (v / v), and cultured at 37°C for 20h (E.coli uses LB culture based on shaking culture at 37°C; E. .faecalis is cultured in MRS based on 37°C), then centrifuged at 8000×g for 20 minutes, poured off the supernatant, resuspended with ultrapure water, continued to centrifuge at 8000×g for 20 minutes, and poured off the supernatant Live bacteria cells, that is, wet bacteria. The wet bacteria were resuspended in 50mg / LDEHP or 10mg / L MEHP aqueous solution, and the final bacterial concentration reached 1g wet bacteria / L, and the blank control was wet bacteria resuspended in ultrapure water without DEHP and MEHP. The volume of each group is 1 mL. ...

Embodiment 3

[0083] Example 3: Lactobacillus plantarum CCFM1019 has no acute toxic side effects on SD rats

[0084] Lactobacillus plantarum CCFM1019 was resuspended in 2% (w / v) sucrose solution, and the cell density was 1.0×10 9 CFU / mL. Take 10 healthy male SD rats with a body weight of about 100 g, and give 2 mL of the suspension solution of this concentration by intragastric administration every day, observe for a week, and record the death and body weight.

[0085] The results of these tests are listed in Table 4. The results showed that the feeding concentration of 1.0×10 9 CFU / mL of Lactobacillus plantarum CCFM1019 had no significant impact on rats, no significant change in body weight, and no death. The appearance of the rats showed no obvious pathological symptoms.

[0086] Table 4 Changes in body weight and death of rats

[0087]

[0088] Note: -: Rats did not die

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses plantarum lactobacillus CCFM1019, its fermented food and its application in preparing medicine. The effect of the bacteria on promoting the excretion of DEHP and MEHP is not only significantly better than the resident flora E. coli and Enterococcus faecalis, but also better than the commercial strain Lactobacillus rhamnosus LGG. Therefore, the Lactobacillus plantarum CCFM1019 of the present invention can be used as an effective means to prevent and alleviate body damage caused by DEHP and MEHP, and does not have the toxic and side effects of drugs. Lactobacillus plantarum CCFM1019 can be used to prepare pharmaceutical compositions and fermented foods for alleviating and preventing the toxicity of DEHP and its metabolites, and has very broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to plant lactobacillus CCFM1019, its fermented food and its application in the preparation of medicines. Background technique [0002] Bis(2-ethylhexyl) phthalate (hereinafter referred to as DEHP), as a plasticizer, can be added to plastics to increase their flexibility and plasticity. Due to its low price, DEHP is widely used in the production of plastic products such as medical equipment and chemical products, and is currently the most widely used plasticizer in my country. In these plastic products, DEHP is mainly combined with other molecules in the form of hydrogen bonds and van der Waals forces rather than covalent bonds, so it is easy to escape during use, thereby migrating to the environment and even the human body. Health hazard. [0003] Studies have shown that the absorption and distribution of DEHP in the human body lasts for about 4-8 hours, and most of them can be c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P39/02A23L29/00C12R1/25
CPCA61P39/02A23L29/065A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/30Y02A50/30
Inventor 陈卫王刚陈潜赵建新张灏
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com