Method for using centrifugal planting to construct tissue engineering bladder

Abstract

The invention provides a method for using centrifugal planting to construct a tissue engineering bladder. The method comprises the steps of using centrifugal planting equipment to prepare a bladder acellular matrix-seed cell suspension liquid structure; placing the bladder acellular matrix-seed cell suspension liquid structure in a centrifugal machine, wherein the centrifugal machine conducts centrifugal running for a specified time at a specified rotation speed; placing a centrifuged bladder acellular matrix in a culture dish, and using the cultured bladder acellular matrix to construct the tissue engineering bladder. The centrifugal planting equipment is constructed, and the proper rotation speed and the centrifugal planting time of the centrifugal machine are sought. Compared with common static planting and dynamic planting modes like pressure perfusion planting and rotary flask planting, centrifugal planting needs fewer cells; meanwhile, through centrifugal force, the seed cells can be uniformly distributed on the inner wall of a support, so that the planting cells enter the bladder acellular matrix, three-dimensional planting is formed, and the prepared tissue engineering bladder more fits actual bladder tissue.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for constructing a tissue engineering bladder by centrifugal planting. Background technique [0002] Tumors, trauma, inflammation, and congenital malformations of the urinary system can all damage the bladder. At present, the use of intestinal repair and reconstruction is often accompanied by a series of complications. Tissue engineering technology provides a new idea to solve this problem. The research content of tissue engineering mainly focuses on the following three aspects: the cultivation of seed cells; the research of scaffold materials; the research on the replacement of various diseased tissues with tissue engineered tissues. [0003] With the progress of research, the repair, improvement and reconstruction of tissue engineering technology have been applied to multiple tissues and organs, such as: skin, blood vessels, nerves, intestines, etc. The re...

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Problems solved by technology

However, the pressure perfusion equipment is usually more complicated, and the perfusion pressure selection is more difficult

At the same time, the pressure may disintegrate the seed cells and destroy the spatial structure of the scaffold material; therefore, pressure perfusion planting has certain limitations and deficiencies.

Rotary bottle planting, through continuous rotation of the culture bottle, to achieve the contact between the planting cells and the scaffold material, to achieve the purpose of uniform planting, but it usually takes a long time and a large amount of cells. In addition, the rotating culture bottle planting scheme cannot achieve The purpose of rapid penetration of seed cells into the scaffold material

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