Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multi-layer holographic reconstruction and focusing strategy-based construction method of spatial distribution of cell group

A technology of spatial distribution and construction method, applied in image analysis, image data processing, instruments, etc., can solve the problems that it is difficult to ensure focus, and it is difficult for observers to understand the spatial distribution of cells

Active Publication Date: 2018-08-03
重庆东渝中能实业有限公司
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the focal planes of the optical microscope are distributed in a very narrow range, and it is difficult to ensure that the observed cells in the field of view are well focused at the same time
Even after repeated focusing operations, it is often difficult for observers to gain an intuitive understanding of the large-scale spatial distribution of cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-layer holographic reconstruction and focusing strategy-based construction method of spatial distribution of cell group
  • Multi-layer holographic reconstruction and focusing strategy-based construction method of spatial distribution of cell group
  • Multi-layer holographic reconstruction and focusing strategy-based construction method of spatial distribution of cell group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0091] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0092] figure 1 Construct a flowchart for the spatial distribution of cell populations based on multilayer holographic reconstruction and focusing strategies, such as figure 1 As shown, the method specifically includes the following steps:

[0093] S1: Read in the hologram to be reconstructed and perform preprocessing.

[0094] Map the hologram from the RGB color space to the corresponding pixel mapping relationship of the grayscale image:

[0095] Gray(i,j)=0.229×R(i,j)+0.587×G(i,j)+0.114×B(i,j)

[0096] Among them, i and j are the row and column coordinates, and R, G, and B are the red, green, and blue channel information of the three primary colors, respectively.

[0097] S2: Set the start reconstruction distance, end reconstruction distance and interlayer step of multi-layer reconstruction; set the reconstruction wavelength lamda ac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a multi-layer holographic reconstruction and focusing strategy-based construction method of spatial field distribution situations of a cell group, and belongs to the field ofdigital holographic imaging application. The method comprises: setting a starting distance and a termination distance of the reconstruction and step length of each layer, carrying out layer-by-layer reconstruction, and recording a reconstruction situation of each layer; carrying out grayscale counting for a reconstruction image of a certain layer, using lognormal distribution to fit curvature extremums of a curve, setting grayscale thresholds, and combining a morphological processing means to extract a target object region; establishing a cell focusing judgment function, and adding informationof depth, at which focusing is best, after tracking and matching of the same cell imaging centroid between adjacent layers to form spatial positions where cells are located; and respectively calculating focusing depth of each cell in a hologram and horizontal-plane centroid projection in a reconstruction image of a corresponding layer to construct spatial distribution of the cell group. The method lays an important foundation for tracking multi-cell movement trajectories by multi-image holographic reconstruction, and is generally suitable for observation and measurement of microscopic non-cell objects.

Description

technical field [0001] The invention belongs to the application field of digital holographic imaging, and relates to a cell space field distribution construction method based on multi-layer holographic reconstruction and object focusing strategies. Background technique [0002] In the actual optical microscopic observation process, since the sample often has a certain thickness, the distribution of cells not only has two-dimensional position information of plane projection, but also has longitudinal depth position information. The strength of cell activity, whether it is apoptotic, or whether it adheres to the wall will cause differences in its spatial distribution. In addition, the focal planes of the optical microscope are distributed in a very narrow range, and it is difficult to ensure that the observed cells in the field of view are well focused at the same time. Even after repeated focusing operations, it is often difficult for observers to gain an intuitive understan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G06T7/11G06T7/136G06T7/155G06T7/30G06T7/66G06T7/50
CPCG06T7/11G06T7/136G06T7/155G06T7/30G06T7/50G06T7/66
Inventor 廖彦剑罗洪艳金变朱子岩叶东海李川李明勇罗小刚
Owner 重庆东渝中能实业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com