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Ror1-positive mesenchymal stem cells and method for preparing same, pharmaceutical composition containing ror1-positive mesenchymal stem cells and method for preparing same, and method for preventing or treating diseases by using ror1-positive mesenchymal stem cells

A technology of mesenchymal stem cells and compositions, which can be used in drug combinations, genetically modified cells, cell culture active agents, etc., can solve the problem that the therapeutic effect of mesenchymal stem cells cannot be said to be sufficient, and achieve excellent mitochondrial transfer ability and excellent therapeutic effect. Effect

Active Publication Date: 2018-07-31
ROHTO PHARM CO LTD
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Problems solved by technology

However, the therapeutic effect of these mesenchymal stem cells cannot be said to be sufficient

Method used

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  • Ror1-positive mesenchymal stem cells and method for preparing same, pharmaceutical composition containing ror1-positive mesenchymal stem cells and method for preparing same, and method for preventing or treating diseases by using ror1-positive mesenchymal stem cells
  • Ror1-positive mesenchymal stem cells and method for preparing same, pharmaceutical composition containing ror1-positive mesenchymal stem cells and method for preparing same, and method for preventing or treating diseases by using ror1-positive mesenchymal stem cells
  • Ror1-positive mesenchymal stem cells and method for preparing same, pharmaceutical composition containing ror1-positive mesenchymal stem cells and method for preparing same, and method for preventing or treating diseases by using ror1-positive mesenchymal stem cells

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preparation example Construction

[0103] [Preparation of ROR1-positive mesenchymal stem cells]

[0104] The method for preparing ROR1-positive mesenchymal stem cells is not particularly limited, and can be prepared, for example, as follows. That is, mesenchymal stem cells are isolated from umbilical cord, adipose tissue, bone marrow and other tissues according to methods known to those skilled in the art and cultured, and ROR1 is isolated by using an anti-ROR1 antibody that specifically binds to ROR1 through a cell sorter, magnetic beads, etc. Positive cells can thus be obtained. In addition, ROR1-positive mesenchymal stem cells can also be obtained by inducing the expression of ROR1 in mesenchymal stem cells by culturing using a medium described later. In the cell cluster obtained by this induction, preferably 70% or more of the cell cluster are ROR1-positive, more preferably 80% or more are ROR1-positive, still more preferably 90% or more are ROR1-positive, and particularly preferably substantially uniform ...

Embodiment

[0199] Next, the present invention will be described more specifically by way of examples, but the present invention is not limited by the following examples.

[0200]

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Abstract

The purpose of the present invention is to provide: new mesenchymal stem cells having excellent therapeutic effect on various diseases, a new pharmaceutical composition containing the mesenchymal stemcells, and a method for preparing the same. The present invention is ROR1-positive mesenchymal stem cells. Preferably, the ROR1-positive mesenchymal stem cells are CD29-, CD73-, CD90-, CD105-, and CD166-positive, and are derived from an umbilical cord or fat.

Description

technical field [0001] The present invention relates to ROR1-positive mesenchymal stem cells and a preparation method thereof, a pharmaceutical composition containing ROR1-positive mesenchymal stem cells and a preparation method thereof, and a method for preventing or treating diseases using ROR1-positive mesenchymal stem cells. Background technique [0002] In recent years, drug development and regenerative medical research using cells or tissues of living organisms are advancing and attracting attention. Among them, ES cells and iPS cells are being rapidly researched as organ regeneration technology or drug discovery and screening tools. On the other hand, cell therapy that isolates and uses somatic stem cells (mesenchymal stem cells) from bone marrow, adipose tissue, umbilical cord, etc., uses adult stem cells in regenerative medicine to repair tissues damaged by diseases, etc. Since it is inherently functional, it has attracted attention and been researched as a more pr...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K35/28A61P1/00A61P1/16A61P1/18A61P3/00A61P9/00A61P19/00A61P35/00A61P43/00
CPCA61K35/28A61P1/00A61P3/00A61P9/00A61P19/00A61P35/00Y02A50/30C12N5/0662C12N2501/998C12N2510/00
Inventor 池山芳史西田浩之津田智博宇野荣子汤本真代须田一真吉野美穗子吴春忠
Owner ROHTO PHARM CO LTD
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