Fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria

Abstract

The invention discloses a fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria. The reagent comprises reagent liquid A and reagent liquid B, wherein the liquid A comprises water, salt, binding protein, specific antibody, fluorescent whitening agent, dissolution promoter, wetting agent and background counterstain, the liquid B comprises water and fluorescein. The fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria has the advantages that the operation is simple, the staining can be completed just by two operation processes; the staining time is short, observation can be conducted within one minute; compared with a traditional microscopy method, the sensibility and the positive rate are higher; compared with a dry chemical ferment method, the result is more visual and accurate, and the situation of mixed type infection can be better solved, the clinical application is more convenient andquicker, and the diagnostic efficiency of vaginitis and cervicitis is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of biomedical diagnosis, and in particular relates to a fluorescent staining reagent for marking leucorrhea and cervical exfoliated cell pathogenic bacteria. Background technique [0002] Vaginitis is a common gynecological disease caused by pathogenic bacteria in clinical practice. According to the different types of pathogenic bacteria, it can be divided into the following three types: bacterial vaginosis, candida vaginitis, and trichomonas vaginitis. Patients with bacterial vaginosis mainly present with increased vaginal discharge with a fishy smell, especially after sexual intercourse, and may be accompanied by mild genital itching or burning sensation. It is mainly caused by endogenous mixed infection caused by the decrease of Lactobacillus in the vagina and the increase of Gardnerella and anaerobic bacteria. Candidal vaginitis manifests as vulvar erythema, edema, and white membranes. The secretion is...

AI Technical Summary

Problems solved by technology

Disadvantages: low positive rate, atypical morphology easily leads to missed diagnosis, large subjective error of inspectors

Disadvantages: Immediate detection of vaginal secretions is required, otherwise the enzyme will lose its activity; the detection process often requires incubation at 37°C, the operation process is a bit cumbersome, and the enzymatic reaction results are not intuitive, which is likely to cause false positives

Disadvantages: Although the detection results of the culture method are highly accurate, due to the slow growth of fungi, the culture often takes days or even weeks to produce results, which often delays the patient's condition

Disadvantages: Specimens need to be detected immediately after collection, otherwise Trichomonas will die easily and be difficult to observe if the time is too long; there are certain technical requirements for inspectors and large subjective errors

The disadvantage is that the judgment value of some indicators needs to be further demonstrated, and some indicators have no clear scientific basis, and the actual clinical application results are not ideal.

Especially for the judgment of mixed infection, there is a big error

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