Glycosylation peptide for adjusting intestinal fermentation and preparing method and application thereof
A technology for regulating intestinal tract and glycosylation, applied in the application, fermentation, function of food ingredients, etc., can solve the problems of reducing the digestibility of protein and carbohydrates, and achieve the improvement of intestinal fermentation endurance, large capital investment, The effect of enhancing probiotics
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Embodiment 1
[0025] Example 1, this example proposes a preparation method of glycosylated grass carp peptide.
[0026] ⑴The treatment of grass carp
[0027] Remove the head, tail and viscera of fresh grass carp, wash it, take the back meat, cut it into pieces, put it in a food-grade packaging bag, and store it in a -20°C refrigerator for later use. Grass carp with a weight of 1.5-2kg / carp can be selected.
[0028] ⑵ Preparation of grass carp peptide
[0029] Add water (m / v=1:4) to the grass carp meat, beating and homogenizing to obtain grass carp homogenate, add 1% neutral protease to the homogenate, and continue enzymolysis at pH 7.0, 54°C for 1 hour; after the end of enzymolysis , the temperature was raised to 80°C and kept for 10 minutes to obtain an enzymolysis solution; after the enzymolysis solution was cooled, it was centrifuged at 5000r / min for 15 minutes, and spray-dried to obtain grass carp peptide powder.
[0030] (3) Preparation of glycosylated grass carp peptide
[0031] A...
Embodiment 2
[0035] ⑴The treatment of grass carp
[0036] Remove the head, tail and viscera of fresh grass carp, wash it, take the back meat, cut it into pieces, put it in a food-grade packaging bag, and store it in a -20°C refrigerator for later use.
[0037] ⑵ Preparation of grass carp peptide
[0038] Grass carp meat was beaten with water (m / v=1:4) and homogenized to obtain grass carp homogenate. Add 1.5% neutral protease to the homogenate, and continue enzymatic hydrolysis at pH 7.0 and 54°C for 1 hour; after the enzymatic hydrolysis, Raise the temperature to 85°C and keep it for 15 minutes to obtain the enzymolysis solution; after the enzymolysis solution is cooled, centrifuge at 6000r / min for 10 minutes, take the supernatant and pass it through a 0.22μm filter membrane, and vacuum freeze-dry to obtain grass carp peptide powder.
[0039] (3) Preparation of glycosylated grass carp peptide
[0040] A. Grass carp peptide powder obtained in step (2) is dispersed into a 50mM phosphate bu...
Embodiment 3
[0043] Example 3, Meal Components
[0044] The glycosylated grass carp peptide prepared in Example 1 (the glycosylated grass carp peptide accounts for 6.5% of the total dietary ingredients) was added to the dietary ingredients to obtain a diet containing glycosylated grass carp peptide.
[0045] After feeding the above-mentioned diet to rats for 3 weeks, the amount of short-chain fatty acids in rat colon was greatly increased (Table 1), showing strong fermentation endurance, while the relative abundance of Bifidobacteria and Lactobacillus in rat feces There was no significant difference with the GOS group, and there was no significant difference in the water content of the feces with the GOS group, indicating that our glycosylated products have similar prebiotic effects to GOS and improved the fermentation tolerance of GOS.
[0046] Table 1 Short-chain fatty acids in intestinal contents of rats (n=8) ingested glycosylated peptides
[0047]
[0048] Note: ** in the table in...
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