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Glycosylated peptide regulating intestinal fermentation, preparation method and application thereof

A technology to regulate intestinal tract and glycosylation, applied in the direction of fermentation, application, and the function of food ingredients, etc., can solve the problems of reducing the digestibility of protein and carbohydrates, and achieve the purpose of improving intestinal fermentation endurance, enhancing probiotic activity, The effect of large capital investment

Active Publication Date: 2021-09-21
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Maillard reaction can reduce the digestibility of protein and carbohydrates in food

Method used

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  • Glycosylated peptide regulating intestinal fermentation, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1, this example proposes a preparation method of glycosylated grass carp peptide.

[0026] ⑴The treatment of grass carp

[0027] Remove the head, tail and viscera of fresh grass carp, wash it, take the back meat, cut it into pieces, put it in a food-grade packaging bag, and store it in a -20°C refrigerator for later use. Grass carp with a weight of 1.5-2kg / carp can be selected.

[0028] ⑵ Preparation of grass carp peptide

[0029] Add water (m / v=1:4) to the grass carp meat, beating and homogenizing to obtain grass carp homogenate, add 1% neutral protease to the homogenate, and continue enzymolysis at pH 7.0, 54°C for 1 hour; after the end of enzymolysis , the temperature was raised to 80°C and kept for 10 minutes to obtain an enzymolysis solution; after the enzymolysis solution was cooled, it was centrifuged at 5000r / min for 15 minutes, and spray-dried to obtain grass carp peptide powder.

[0030] (3) Preparation of glycosylated grass carp peptide

[0031] A...

Embodiment 2

[0035] ⑴The treatment of grass carp

[0036] Remove the head, tail and viscera of fresh grass carp, wash it, take the back meat, cut it into pieces, put it in a food-grade packaging bag, and store it in a -20°C refrigerator for later use.

[0037] ⑵ Preparation of grass carp peptide

[0038] Grass carp meat was beaten with water (m / v=1:4) and homogenized to obtain grass carp homogenate. Add 1.5% neutral protease to the homogenate, and continue enzymatic hydrolysis at pH 7.0 and 54°C for 1 hour; after the enzymatic hydrolysis, Raise the temperature to 85°C and keep it for 15 minutes to obtain the enzymolysis solution; after the enzymolysis solution is cooled, centrifuge at 6000r / min for 10 minutes, take the supernatant and pass it through a 0.22μm filter membrane, and vacuum freeze-dry to obtain grass carp peptide powder.

[0039] (3) Preparation of glycosylated grass carp peptide

[0040] A. Grass carp peptide powder obtained in step (2) is dispersed into a 50mM phosphate bu...

Embodiment 3

[0043] Example 3, Meal Components

[0044] The glycosylated grass carp peptide prepared in Example 1 (the glycosylated grass carp peptide accounts for 6.5% of the total dietary ingredients) was added to the dietary ingredients to obtain a diet containing glycosylated grass carp peptide.

[0045] After feeding the above-mentioned diet to rats for 3 weeks, the amount of short-chain fatty acids in rat colon was greatly increased (Table 1), showing strong fermentation endurance, while the relative abundance of Bifidobacteria and Lactobacillus in rat feces There was no significant difference with the GOS group, and there was no significant difference in the water content of the feces with the GOS group, indicating that our glycosylated products have similar prebiotic effects to GOS and improved the fermentation tolerance of GOS.

[0046] Table 1 Short-chain fatty acids in intestinal contents of rats (n=8) ingested glycosylated peptides

[0047]

[0048] Note: ** in the table in...

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Abstract

The present invention proposes a method for preparing a glycosylated peptide that regulates intestinal fermentation, including the following steps: (1) Preparation of fish peptide powder: take fish, add water, beat, and homogenize to obtain a fish homogenate, and add neutral protease to the homogenate , heated to 40-60°C, and continued to enzymatically hydrolyze for 1-5h; after the end of the enzymatic hydrolysis, the temperature was raised to 80-90°C and kept for 10-20min to obtain the enzymatic hydrolysis solution; after the enzymatic hydrolysis solution was cooled, centrifuge and take the supernatant After filtration, spray-dry to obtain fish peptide powder; (2) preparation of glycosylated peptide: A, disperse the fish peptide powder obtained in step (1) into a buffered saline solution with a concentration of 1%-5%, and add 6%-15% of Polysaccharides are mixed evenly and then reacted in a water bath to obtain a mixed solution; B, the mixed solution obtained in step A is concentrated in vacuum to obtain a concentrated solution; C, the concentrated solution obtained in step B is dried to obtain a glycosyl peptide products. The invention makes full use of biological resources such as fish to produce an active product with intestinal regulation, which can be applied to dietary ingredients and can effectively regulate animal or human intestinal fermentation.

Description

technical field [0001] The invention belongs to the field of food technology, and in particular relates to a glycosylated peptide for regulating intestinal fermentation, a preparation method and application thereof. Background technique [0002] Enzymatic hydrolysis of grass carp protein to prepare grass carp peptide can effectively utilize my country's rich freshwater fish resources and will produce huge economic and social benefits. Therefore, deep processing of grass carp is feasible and practical. [0003] Galacto-oligosaccharides (GOS) are 1-7 galactosyl groups connected to the galactosyl side of lactose by β-1,4 glycosidic bonds. It is stable in nature and has the ability to reduce the incidence of dental caries, promote the proliferation of intestinal bifidobacteria, Preventing constipation, improving mineral absorption, regulating lipid metabolism and other physiological functions, it is a functional oligosaccharide with high safety and the most widely recognized, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A23L33/18
CPCA23V2002/00A23L33/18C12P21/06A23V2200/32
Inventor 董士远靳卫亚杨宇鸿曾名湧吴浩浩刘尊英赵元晖
Owner OCEAN UNIV OF CHINA
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