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Primordial follicle activator and its application in culture medium of human ovarian cortex

An ovarian cortex and culture medium technology, applied to primordial follicle activator and its application in human ovarian cortex culture medium, can solve the problems of complex types of activators, shorten activation time, etc., and achieve the effect of efficient, fast and convenient application

Inactive Publication Date: 2021-06-18
南京艾维康泰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the types of activators involved in the above-mentioned patents are too complicated, and the in vitro treatment time takes 48 hours, the patient must undergo two operations to complete the treatment process in clinical application. Therefore, how to find a new activation method to shorten the activation time , so that the treatment can be completed through one operation, reducing the pain of the patient, and the operation is convenient and quick, which will be a revolutionary leap in this technology

Method used

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  • Primordial follicle activator and its application in culture medium of human ovarian cortex
  • Primordial follicle activator and its application in culture medium of human ovarian cortex
  • Primordial follicle activator and its application in culture medium of human ovarian cortex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Novel human ovarian cortex in vitro culture medium, including the following components:

[0027] Wherein, the solution I preparation method is as follows:

[0028] Basic medium: MEMa (GIBCO TM Minimum Essential Medium (MEM) Alpha Medium (1X) liquid, Invitrogen) was used as mother liquor, and human serum albumin 10wt.%, sodium pyruvate 0.23mM was added;

[0029] Wherein, reagent I preparation method is as follows:

[0030] mTOR signaling pathway activator I, containing 100μM phosphatidic acid, prepackaged according to the dose of 30mL basic activated cell culture medium;

[0031] Wherein, reagent II preparation method is as follows:

[0032] PI3K signaling pathway activator II, containing 1000μg / mL 740Y-P, pre-packed according to the dose of 30mL basic activated cell culture medium;

[0033] The above two activators were prepackaged into small packaging boxes, and then packaged together with solution I into a larger packaging box, and operated according to the human...

Embodiment 2

[0047] mTOR signaling pathway activator, mTOR signaling pathway activator I is 50-200 μ M phosphatidic acid (phosphatidic acid,

[0048] PA), the phosphatidic acid is 100 μM.

[0049] The application of the mTOR signaling pathway activator in the preparation of novel human ovarian cortex culture fluid.

[0050] PI3K signaling pathway activator, PI3K signaling pathway activator II, is 1000 μg / mL 740Y-P (740Y-P is a polypeptide, its amino acid sequence is: RQIKIWFQNRRMKWKKSDGGYMDMS (Modifications: Tyr-25=pTyr)).

[0051] The combined application of the PI3K signal pathway activator and the mTOR signal pathway activator in the preparation of novel human ovarian cortex culture fluid.

[0052] The new human ovarian cortex culture medium includes the following components:

[0053] 1) mTOR signaling pathway activator I;

[0054] 2) PI3K signaling pathway activators I and II;

[0055] 3) Basic culture medium.

[0056] The composition scheme of the culture solution is as follows: ...

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Abstract

Primitive follicle activator and its application in human ovarian cortex culture fluid, composed of mTOR signaling pathway activator and PI3K signaling pathway activator, said mTOR signaling pathway activator includes mTOR signaling pathway activator I, mTOR signaling pathway activator I is 50-200 μM phosphatidic acid; the PI3K signaling pathway activator includes PI3K signaling pathway activator II, and the PI3K signaling pathway activator II is 100-1000 μg / mL 740Y-P. The development of this new type of culture medium is more conducive to the clinical promotion of in vitro activation of primordial follicles, expands the scope of treatment indications, and is widely used in the field of assisted reproduction to treat diseases caused by insufficient follicle reserves such as premature ovarian failure and low ovarian function.

Description

technical field [0001] The invention belongs to the technical field of in vitro culture of ovarian tissue, and in particular relates to a primordial follicle activator capable of promoting the development of primordial follicles and its application in human ovarian cortex culture fluid. Background technique [0002] In mammals, germ cell growth and maturation is a process finely regulated by hormones. These hormones include gonadotropins from the hypothalamus and hormones and growth factors produced by the reproductive organs themselves. In female mammals, the follicle is the basic functional unit of the ovary, consisting of an egg in the center and one or more layers of granulosa cells on the periphery. According to the different growth and development stages of oocytes in follicles, they can be divided into primordial follicles, primary follicles, secondary follicles, antral follicles, and preovulatory follicles. Primordial follicles are resting follicle pools in the ova...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075
CPCC12N5/0682C12N2500/30C12N2501/727
Inventor 李晶吴庭鹤张婧陈永生
Owner 南京艾维康泰生物技术有限公司
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