An exopolysaccharide-producing space Lactobacillus plantarum ss18-33 and its application in improving biological antioxidant activity

A technology for Lactobacillus plantarum, exopolysaccharide, applied in the direction of Lactobacillus, microorganism-based methods, biochemical equipment and methods, etc.

Active Publication Date: 2019-12-31
FULLARTON BIOENG TECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign scholars' research on space microorganisms mainly focuses on space pathogens, space corrosion bacteria and microbial pharmaceuticals, while the research on space food microbial engineering bacteria is in a blank state.

Method used

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  • An exopolysaccharide-producing space Lactobacillus plantarum ss18-33 and its application in improving biological antioxidant activity
  • An exopolysaccharide-producing space Lactobacillus plantarum ss18-33 and its application in improving biological antioxidant activity
  • An exopolysaccharide-producing space Lactobacillus plantarum ss18-33 and its application in improving biological antioxidant activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1, to the screening of the space Lactobacillus plantarum strain of high yield EPS

[0071] (1) Isolation and purification of bacterial strains

[0072] 1. Strains

[0073] Space Lactobacillus plantarum (Lactobacillus plantarum) SS18: Lactobacillus plantarum (Lactobacillus plantarum) GS18 (General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation number: CGMCC 1.485) returned by Tiangong-2 and Shenzhou-11 spacecraft Space mutation strains are preserved in the Space Microbial Strain Bank of Fullerton Bioengineering Technology (Beijing) Co., Ltd. In order to distinguish the difference between the strains before and after the space launch, the space-mutated strain after the ground-borne Lactobacillus plantarum (Lactobacillus plantarum) GS18 was recorded as the space Lactobacillus plantarum (Lactobacillus plantarum) SS18.

[0074] 2. Strain activation

[0075] Put the glycerol preservation tubes of ground Lactoba...

Embodiment 2

[0110] Example 2, detection of antioxidant activity of EPS produced by Lactobacillus plantarum SS18-33 in space

[0111] (1) Preparation of space Lactobacillus plantarum SS18-33 starter

[0112] Activate the Lactobacillus plantarum SS18-33 in the MRS liquid medium to obtain the activated MRS test tube culture, and transfer the activated MRS test tube culture into a 100mL sterile MRS container with a 3% (volume percentage) inoculum. In the Erlenmeyer flask of the liquid medium, after culturing at 37° C. for 16 hours, the starter of Lactobacillus plantarum SS18-33 was obtained. The number of live bacteria of Lactobacillus plantarum SS18-33 in the starter is (2.0~5.0)×10 9 CFU / mL.

[0113] (2) Fermentation test of Lactobacillus plantarum SS18-33 producing EPS

[0114] Put Lactobacillus plantarum SS18-33 starter into 1000mL liquid MRS liquid medium (initial pH of the medium is 7.0) according to 3% (volume percentage) inoculation amount, and carry out EPS production fermentation...

Embodiment 3

[0152] Embodiment 3, space Lactobacillus plantarum SS18-33 bacterial strain resistance gastrointestinal adverse environment test

[0153] Artificially simulated gastric juice: containing NaCl 0.2g / 100mL, pepsin (enzyme activity: 250U / mg) 0.3g / 100mL, adjusted pH to 3.0 with 1mol / L HCl, the balance was deionized water, filtered through a 0.22μm filter membrane Reserve after sterilization.

[0154] Artificially simulated intestinal juice: dissolve trypsin (enzyme activity: 1655U / mg) in 0.1mol / L phosphate buffer (pH8.0) to make the final concentration 1g / L, add ox bile salt to make the concentration 0.3g / 100mL, filter and sterilize through a 0.22μm membrane filter before use.

[0155] Strains to be tested: Lactobacillus plantarum GS18 on the ground and Lactobacillus plantarum SS18-33 in space.

[0156] 1. Inoculate the strain to be tested in 10mL MRS liquid medium (pH 7.0), and culture at 37°C for 18 hours (thalline concentration is 2.0×10 9 CFU / mL~8.0×10 9 CFU / mL), centrifuge...

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Abstract

The invention discloses an exopolysaccharide-producing space lactobacillus plantarum SS18‑33 and its application in improving biological antioxidant activity. The preservation number of the space Lactobacillus plantarum SS18-33 disclosed by the present invention in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.15151. The exopolysaccharide produced by the space lactobacillus plantarum SS18-33 of the present invention has DPPH scavenging ability, superoxide anion free radical (O 2 ‑ ·) scavenging ability, for Fe 2+ Chelating ability and the total reducing ability or total antioxidant ability to block the formation of peroxides by providing hydrogen atoms; in addition, the strain also has the characteristics of gastrointestinal adverse environment tolerance, which contributes to the improvement of biological antioxidant activity of exopolysaccharides The application in provides a practical basis. The invention fills up the research blank of space food microbial engineering bacteria.

Description

technical field [0001] The invention relates to an exopolysaccharide-producing space lactobacillus plantarum SS18-33 and its application in improving biological antioxidant activity in the field of biotechnology. Background technique [0002] Lactic acid bacteria exopolysaccharides (exopolysaccharides, EPS) are the mucus polysaccharides or capsular polysaccharides secreted by lactic acid bacteria on the cell surface to resist adverse environmental factors under suboptimal growth conditions. 4 ~6.0×10 6 Da between. In the natural environment, EPS usually has the function of protecting microbial cells, such as preventing cell desiccation and dehydration, preventing macrophage invasion or phage infection, and resisting antibiotics or toxic substances. It can also stabilize osmotic pressure, participate in cell information transmission and cell composition etc. Many studies have shown that EPS has immunomodulatory effects, anti-lipid peroxidation activity, anti-tumor effect o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04A23L33/135A23L29/10A23L29/269A61K35/747A61P39/06A61P3/06A61P37/04A61P1/16A61P35/00A61P1/00C12R1/25
CPCA23L29/10A23L29/269A23L33/135C12N1/20C12P19/04A61K2035/115A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/30A23V2200/32A23V2200/308
Inventor 郝红炜刘慧张红星谢远红
Owner FULLARTON BIOENG TECH BEIJING CO LTD
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