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Dropping device for making bone marrow chromosome G-band

A technology for chromosomes and droplets, applied in the field of biomedical testing, can solve problems such as no relevant reports, and achieve the effects of improving quality, realizing standardized operations, and reducing fatigue injuries.

Active Publication Date: 2018-07-24
济南金域医学检验中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the above-mentioned dropping devices for chromosome specimens meet the requirements for the height of the dropping sheet during karyotype analysis by improving the distance adjustment method between the glass slide and the dropper mouth, and for the turbidity control of the cell suspension before dropping the sheet, As well as the over-fire operation after dropping the sheet, the above-mentioned devices for dropping the chromosome sample are not involved, and there are no relevant reports in the prior art

Method used

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  • Dropping device for making bone marrow chromosome G-band

Examples

Experimental program
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Embodiment 1

[0039] A droplet device for making bone marrow chromosome G band, its structure is as follows figure 1 As shown, it includes: a turbidimetric unit 1 , a droplet unit 2 , an overheated unit 3 and a cooling unit 4 connected in sequence.

[0040] The structure of the turbidimetric unit 1 is as follows figure 2 As shown, it includes a base 11, 2 McFarland standard tubes 13, 3 McFarland standard tubes 14 and a sample tube 15; the base 11 is provided with a hole 12, The McFarland standard tube and the sample tube are placed in the hole; the base 11 is also provided with a white board 16, which is convenient for comparing the sample tube and the McFarland standard tube.

[0041] The structure of the drip unit 2 is as image 3 As shown, it includes a drip table 21 and a drip device; the table of the drip table 21 is provided with a protrusion 22, and the height of the protrusion 22 is 1.0 cm higher than the table of the drip table; the drip device includes a vertical The telescopi...

Embodiment 2

[0054] Utilize the dripping device of embodiment 1 to carry out the making of bone marrow chromosome G band, the steps are as follows:

[0055] (1) Collect the bone marrow cell culture, prepare it into a cell suspension, add it to the sample tube of the turbidimetric unit, compare the sample tube with the McFarland standard tube of the turbidimetric unit, and adjust the concentration of the cell suspension, Make the turbidity of the cell suspension in the sample tube between 2 McFarland turbidity and 3 McFarland turbidity;

[0056] (2) Place the pre-cooled and clean glass slide on the drop slide table, place one end of the slide on the protrusion of the drop slide table, use a dropper to draw the cell suspension with adjusted turbidity in the sample tube, and put the dropper It is fixed on the dropper card position of the dropper unit, and the height of the telescopic rod of the dripping device is adjusted to make the drop height 8-10cm. The dropper is moved in the horizontal ...

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Abstract

The invention discloses a dropping sheet device for making a bone marrow chromosome G-band. The dropping device comprises a specific turbid unit, a dropping unit, a heating burning unit and a normal-temperature cooling unit. For the dropping device provided by the invention, by arranging the specific turbid unit, the turbidity of cell suspension of a bone marrow cell culture is regulated and is controlled at 2-3 Macfarland turbidity, so that a division phase of chromosome can be prevented from becoming scattered, the phenomenon that insufficient burning is prevented, and the mass of a preparedbone marrow chromosome specimen is improved; and by arranging the burning unit, a glass slide can be uniformly burnt, and staff is prevented from being burnt during burning operation. The dropping device provided by the invention can realize standard operation of bone marrow chromosome dropping and lower the fatigue and damage of the staff caused by dropping.

Description

technical field [0001] The invention relates to the technical field of biomedical testing, in particular to a droplet device for making bone marrow chromosome G bands. Background technique [0002] Bone marrow karyotype analysis plays an important role in the diagnosis, treatment and prognosis of blood system diseases. In the process of chromosome karyotype analysis, the quality of staining of bone marrow chromosome specimen directly affects the result analysis. In the staining process, the dropping operation is a very important link, which directly affects the success or failure of the karyotype analysis. [0003] Existing instruments for making chromosome droplets are not perfect, and often need to be manually carried out one by one, so it is easy to cause a situation that the supply exceeds demand when a large number of droplets are needed. Moreover, manual dripping requires one hand to hold the slide, or place the slide on the table, and the other hand to hold the drop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/31G01N1/44G01N21/82
CPCG01N1/312G01N1/44G01N21/82
Inventor 谢风祥王婷婷吴晓飞王丙维彭德志王佳佳韦杰
Owner 济南金域医学检验中心有限公司
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