A method for producing lycopene by B. trispora fermentation and lycopene

Abstract

The invention relates to a method for producing lycopene by B. trispora fermentation and lycopene, belonging to the field of fermentation engineering. The above method comprises: respectively cultivating B. trispora positive bacteria and B. trispora negative bacteria, taking part of the cultivated B. trispora positive bacteria and B. trispora negative bacteria to inoculate simultaneously, fermenting, and controlling the fermentation system with a regulator during the fermentation process. The pH value is 5.8-6.2, feeding is carried out during 40-100h after the fermentation starts, the fermentation is continued until 120h, and the tank is put. Regulator is containing PO 4 3+ And does not contain acidic substances of metal ions and salts. The fermentation medium contains starch phosphates. This method is simple and low cost, by using PO 4 3+ And acidic substances without metal ions and salts control the fermentation pH and provide PO 4 3+ , to avoid bringing in excess K + , coupled with the use of starch phosphates to increase the production of lycopene. The resulting lycopene has a relatively high yield and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of fermentation engineering, and in particular to a method for producing lycopene by B. trispora fermentation and lycopene. Background technique [0002] Existing fermentation methods usually use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ salt to increase phosphorylation levels and provide K + , enhance the permeability of cell membrane, and benefit the entry of nutrients into cells. Use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ If the amount of salt is too small, the phosphorylation level will be insufficient, which will greatly reduce the metabolic flux of carotenoids, and if it is too much, it will bring a large amount of K + , so that the cell membrane permeability of the bacteria becomes too strong, resulting in the precipitation of internal substances such as the target product lycopene into the fermentation clear night. [0003] At the same time, due to the need to add an alkaline blocking agent during t...

AI Technical Summary

Problems solved by technology

Use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ If the amount of salt is too small, the phosphorylation level will be insufficient, which will greatly reduce the metabolic flux of carotenoids, and if it is too much, it will bring a large amount of K + , so that the cell membrane permeability of the bacteria becomes too strong, resulting in the precipitation of internal substances such as the target product lycopene into the fermentation clear night

[0003] At the same time, due to the need to add an alkaline blocking agent during the fermentation process, it inhibits the production of the acidic hormone trisporic acid by B. trispora, which further leads to the weakening of the metabolic flux of lycopene. precursor substances such as β-ionone, etc., but the added precursor substances are usually expensive, or difficult to obtain from the market in large quantities, these are the defects of its application in industrial production

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