Hematoxylin-eosin rapid staining solution and application method

A rapid dyeing and hematoxylin technology, applied in the field of hematoxylin-eosin rapid staining solution, can solve the problems of unclear outline, flaky white areas, opacity, light coloring of cell nuclei, etc., and achieve the effect of shortening the staining time

Inactive Publication Date: 2018-07-13
南京福怡科技发展股份有限公司
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The nuclei are lightly stained, with unclear outlines and varying degrees of flaky white areas;
[0005] 2. The cytoplasm is lightly colored, or even not colored;
[0006] 3. Nucleoplasmic co-staining, fuzzy staining, and unclear tissue structure;
[0007] 4. The section is unevenly stained, and there are spots of poorly colored areas;
[0008] 5. The slice is not clear and opaque;
[0009] 6. Section staining is not bright, fuzzy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hematoxylin-eosin rapid staining solution and application method
  • Hematoxylin-eosin rapid staining solution and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Improved hematoxylin staining solution 1, the components of each liter of staining solution are: hematoxylin color essence 2g, aluminum potassium sulfate dodecahydrate 6g, sodium iodate 0.06g, methanol 150ml, absolute ethanol 650ml, sodium sulfate 25ml, purified water 173ml .

[0031] New-type eosin staining solution 1, the components of each liter of dyeing solution are: eosin Y alcohol-soluble 6.5g, eosin Y water-soluble 0.8g, orange G 0.6g, sodium sulfate 40ml, ammonia water 90ml, absolute ethanol 800ml, purified water 68ml.

[0032] The staining steps of the staining solution are:

[0033] 1) Paraffin sections were dewaxed twice, each time for 2 minutes at room temperature;

[0034] 2) Absolute ethanol 1 time, 2 minutes;

[0035] 3) Place the slices in the hematoxylin staining solution for 1 minute;

[0036] 4) Take out the slide and wash with water for 30 seconds;

[0037] 5) Place the slice in the new eosin staining solution for 5 seconds;

[0038] 6) Take o...

Embodiment 2

[0042] Improved hematoxylin staining solution 2, the components of each liter of the staining solution are: hematoxylin 1g, iron alum 2g, potassium permanganate 0.03g, methanol 100ml, absolute ethanol 600ml, glacial acetic acid 20ml, purified water 278ml.

[0043] New-type eosin staining solution 2, the components of each liter of dyeing solution are: eosin Y alcohol-soluble 6.0g, eosin Y water-soluble 0.03g, orange G 0.3g, glacial acetic acid 20ml, ammonia water 40ml, absolute ethanol 800ml, purified water 136ml.

[0044] The staining steps of the staining solution are:

[0045] 1) Paraffin sections were dewaxed twice, heated at 37°C for 1 minute each time;

[0046] 2) Absolute ethanol 1 time, 1 minute;

[0047] 3) Place the slices in the hematoxylin staining solution for 1.5 minutes;

[0048] 4) Take out the slide and wash with water for 30 seconds;

[0049] 5) Place the slice in the new eosin staining solution for 5 seconds;

[0050] 6) Take out the slices and dehydrat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a hematoxylin-eosin rapid staining solution and an application method thereof, which belong to the technical field of biology. Specifically speaking, the hematoxylin-eosin rapid staining solution is prepared from an improved hematoxylin staining solution and a novel eosin staining solution. The hematoxylin-eosin rapid staining solution provided by the invention is easy to operate, staining is uniform, a stained cellular structure is clear, cell coloration is vivid, and clinical pathological diagnosis, observation and judgment are facilitated.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid hematoxylin-eosin staining solution, and simultaneously the invention also relates to an application method of the staining solution. Background technique [0002] Hematoxylin-eosin staining, referred to as HE staining, is the most common and widely used conventional staining method for pathological tissue sections. Hematoxylin is easily oxidized, and its oxidation product hematoxylin is the real fuel. It is customary to say that hematoxylin is a basic dye, but in fact hematoxylin is an acid dye, and only a positively charged blue color essence formed by hematoxylin and aluminum is alkaline. The combination of the positively charged blue color essence and the nucleus is accomplished through the polar adsorption of positive and negative charges, that is to say, the negatively charged deoxyribose nucleic acid root and the positively charged blue color essence are completed by ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C09B67/22C09B67/44G01N1/30
CPCC09B67/0033C09B67/0083G01N1/30G01N2001/302
Inventor 姚斌左露露
Owner 南京福怡科技发展股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap