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A preparation for promoting skin wound healing and its preparation method and application

A wound healing and preparation technology, applied in the field of preparations and preparations for promoting skin wound healing, can solve the problems of long repair time and secondary injury of patients, and achieve the effects of improving regeneration ability, promoting repair and promoting wound healing.

Active Publication Date: 2021-01-05
广东龄值生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a preparation for promoting skin wound healing and its preparation method and application, which are used to solve the problems that the current wound treatment methods take a long time to repair or cause secondary damage to patients

Method used

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  • A preparation for promoting skin wound healing and its preparation method and application
  • A preparation for promoting skin wound healing and its preparation method and application

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preparation example Construction

[0031] The present invention also provides a preparation method for the preparation of the technical solution, including:

[0032] a) uniformly mixing the umbilical cord stem cell conditioned medium and Matrigel at 0-4°C to obtain a first mixed solution;

[0033] b) resuspending endothelial progenitor cells in physiological saline containing vascular endothelial factor to obtain a second mixed solution;

[0034] c) mixing the first mixed liquid and the second mixed liquid at 0-4°C, and then placing them in a mold to form a preparation.

[0035] In the present invention, the volume ratio of the first mixed liquid to the second mixed liquid is 1:1-3.

[0036] In the present invention, the umbilical cord stem cell conditioned medium is obtained as follows:

[0037] The umbilical cord stem cells are subcultured to obtain the P2-P5 generation umbilical cord stem cells, and the culture supernatant of the P2-P5 generation umbilical cord stem cells is collected to obtain the umbilic...

Embodiment 1

[0045] Isolation and Extraction of Endothelial Progenitor Cells

[0046] In this example, Ficoll gradient centrifugation was used to separate living cells from human umbilical cord blood mononuclear cells, combined with MACS magnetic bead antibody labeling method to sort CD133 + Cells to remove most of the blood cells and platelets, etc., and then remove mature endothelial cells and monocytes by the adherence method, and use the attachment performance of endothelial progenitor cells to remove other cells to obtain endothelial progenitor cells.

Embodiment 2

[0048] Collection of umbilical cord stem cell conditioned media

[0049] Take umbilical cord stem cells of P2-P3 generation with a confluence of 80%-90%, wash them with PBS for 1-3 times, and add 0.015-0.04mL / cm2 to the cells 2 0.05% to 0.3% trypsin + 0.01% to 0.04% EDTA to digest for 1 to 3 minutes, use 5 to 10 times the complete medium of the digestion solution to stop the enzymolysis, centrifuge at 200 to 400g for 5 minutes, and resuspend with the complete medium , inoculated in a culture dish or a culture bottle, the passage density is 8000-15000cell / cm 2. After culturing for 24 hours, replace the phenol red-free 1640 basal medium. After the cells continued to culture for 48 hours, collect the culture supernatant, centrifuge at 200-400 g for 5 minutes, take the supernatant, and concentrate the volume to 50 mL to obtain the umbilical cord stem cell conditioned medium. The umbilical cord stem cell conditioned medium was stored at 4°C for later use. Among them, the volume ...

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Abstract

The invention belongs to the field of medicine products and particularly relates to a preparation for promoting healing of a skin wound as well as a preparation method and application of the preparation. The invention provides the preparation which is prepared from endothelial progenitor cells, vascular endothelial factors, an umbilical cord stem cell conditioned medium and matrix gelatin. Experimental results show that the preparation can promote healing of the skin wound. After the preparation provided by the invention is applied, the cell factors and the endothelial progenitor cells in thepreparation are released, so that formation of regenerated tissues is promoted, and the effect of transplanting repair is exerted; and the umbilical cord stem cell conditioned medium contains variousactive factors, so that the regeneration capacity of tissues can be improved, repair of original tissues is promoted, and the effect of omni-directionally promoting healing of the wound is exerted.

Description

technical field [0001] The invention belongs to the field of medical products, and in particular relates to a preparation for promoting skin wound healing, a preparation method and application thereof. Background technique [0002] The skin is an important organ of the human body and has various functions such as barrier protection, absorption and metabolism, secretion and excretion, temperature regulation, and immunity. Due to trauma and other reasons, it may cause extensive damage to the skin, and may cause complications and endanger human life. The wound healing process is divided into four phases: (1) Interstitial fluid exudation phase: collagen fibrous cells secrete collagen to fill the wound, and type I collagen is the main collagen for scar formation. (2) Inflammatory phase (debridement phase): it occurs within 24-48 hours and is accompanied by bacterial infection. At this time, immune cells mainly clear damaged tissues and microorganisms. (3) Proliferation and diff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61P17/02A61K35/51A61K35/44
CPCA61K35/44A61K35/51A61K38/1866A61K2300/00
Inventor 马颖
Owner 广东龄值生物科技有限公司
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