Multi-membrane western blot hybridization instrument and its experimental operation method

Abstract

The invention relates to the field of Western blot experimental equipment and specifically relates to a multi-membrane Western blot hybridization instrument and an experimental operation method thereof. The instrument comprises a bottom shell and a vertical shell, wherein a transfer positioning device is arranged in the vertical shell; a shaking table device is arranged in the bottom shell; a hybridization trough group is arranged at the upper part of the shaking table device; the hybridization trough group comprises: an independent closed liquid trough, an anti-hybridization trough I, an elution trough and an anti-hybridization trough II; and a liquid inlet / discharge device is arranged on the elution trough. A PVDF membrane after membrane transfer is immersed in the closed liquid trough,the anti-hybridization trough I, the elution trough and the anti-hybridization trough II sequentially through the transfer positioning device, and then returns to the elution trough to be subjected tothe hybridization process. With the scheme, according to the multi-membrane Western blot hybridization instrument and the experimental operation method thereof provided by the invention, the instrument structure is simple, the experimental operation is convenient, simultaneous experiments on multiple membranes are supported, the working strength of experimenters can be relieved, the working efficiency is improved, and the experimental cost is lowered.

Description

technical field [0001] The invention relates to the technical field of western blot, in particular to a multi-membrane western blot hybridization instrument and an experimental operation method thereof. Background technique [0002] Western blot hybridization (Western blot) is one of the most commonly used techniques in biological experiments. The experimental operation is mainly based on gel electrophoresis, and the protein components separated by electrophoresis are transferred from the gel to a solid support. , the process of specific immunoreaction between the antibody and the antigenic epitope presented by the target protein attached to the solid support, followed by staining and detection. [0003] Among them, the protein hybridization process that allows the antibody to have a specific immune reaction with the target protein can be subdivided into three steps: blocking, hybridization incubation, and elution. The PVDF (polyvinylidene fluoride) membrane after electropho...

AI Technical Summary

Problems solved by technology

However, such instruments and consumables are very expensive, even for laboratories with more scientific research funds, the related costs are difficult to bear; moreover, most instruments can only perform hybridization experiments on a single PVDF membrane, and the experimental efficiency is limited. Low, unable to meet the experimental needs of some experiments that require simultaneous experiments with multiple diaphragms. The scope of application is narrow, and the instrument that supports simultaneous multi-diaphragm experiments will increase the price of the instrument again on top of the already expensive price.

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