Production method of low-ash-content soybean peptide containing probiotics
A production method and technology of soybean peptides, which are applied in the directions of food ingredients, functions of food ingredients, protein food ingredients, etc., can solve problems such as unpleasant taste of products, and achieve the effect of improving intestinal health.
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Embodiment 1
[0036] The production method of the low-ash soybean peptide containing probiotics comprises the following steps:
[0037] (1) Add water to the soybean protein isolate to prepare a solution with a mass concentration of 10%, then adjust the pH to 8.0 and the temperature at 55°C, and add alkaline protease and 0.5% of the flavor of the soybean protein isolate at a ratio of 1.0% of the dry weight of the soybean protein isolate Protease, after 6 hours of enzymatic hydrolysis reaction, adjust the pH to 4.0 to prepare soybean peptide reaction solution;
[0038] (2) Microfiltration the soybean peptide reaction solution prepared in step (1) through a microfiltration membrane with a pore size of 1 μm to remove unhydrolyzed protein to obtain a microfiltrate, and then add activated carbon to the microfiltrate in a proportion of 1% by mass , at a temperature of 55°C and a pH of 4.2, decolorize for 90 minutes to obtain a decolorized feed solution. After testing, the light transmittance of th...
Embodiment 2
[0048] The production method of the low-ash soybean peptide containing probiotics comprises the following steps:
[0049] (1) Soybean protein isolate is mixed with water to form a solution with a mass concentration of 8%, then adjust the pH to 7.5 and the temperature at 55°C, and add alkaline protease and 0.6% of the local flavor according to the dry weight of soybean protein isolate Protease, after 8 hours of enzymolysis reaction, adjust the pH to 4.5 to prepare soybean peptide reaction solution;
[0050](2) Microfiltration the soybean peptide reaction solution prepared in step (1) through a microfiltration membrane with a pore size of 1 μm to remove unhydrolyzed protein to obtain a microfiltrate, and then add activated carbon to the microfiltrate in a proportion of 1% by mass , at a temperature of 60°C and a pH of 4.5, decolorize for 120 minutes to obtain a decolorized feed solution. After testing, the light transmittance of the feed liquid is 90%;
[0051] (3) the decolori...
Embodiment 3
[0060] The production method of the low-ash soybean peptide containing probiotics comprises the following steps:
[0061] (1) Soy protein isolate is mixed with water to make a solution with a mass concentration of 10%, then adjust the pH to 8.5, and the temperature is 60°C, and add alkaline protease and 0.4% flavor according to the ratio of 1.5% of the dry weight of soybean protein isolate Protease, after 7 hours of enzymatic hydrolysis reaction, adjust the pH to 4.2 to prepare soybean peptide reaction liquid;
[0062] (2) Microfiltration the soybean peptide reaction solution prepared in step (1) through a microfiltration membrane with a pore size of 1 μm to remove unhydrolyzed protein to obtain a microfiltrate, and then add activated carbon to the microfiltrate in a proportion of 1% by mass , at a temperature of 60°C and a pH of 5, decolorize for 100 minutes to obtain a decolorized feed solution. After testing, the light transmittance of the feed liquid is 87%;
[0063] (3) ...
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