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Kit for measuring digoxin content by magnetic particle chemiluminescence method and its detection method

A chemiluminescence, digoxin technology, used in measurement devices, biological tests, scientific instruments, etc.

Active Publication Date: 2020-07-07
TAIZHOU ZECEN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to overcome the defect that the existing digoxigenin kits cannot be used for both solid-phase coupling and luminescence-labeled digoxin derivatives, and provide a method for the determination of digoxigenin by magnetic particle chemiluminescence. High Xin content kit and detection method thereof

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  • Kit for measuring digoxin content by magnetic particle chemiluminescence method and its detection method

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Experimental program
Comparison scheme
Effect test

Embodiment

[0043] 1. Preparation process of digoxin antigen derivatives:

[0044] 1) Prepare 0.1M HCl solution;

[0045] 2) Prepare 0.01M NaHCO3 buffer with PH=9.0 and 0.15M NaHCO3 buffer with PH=9.5;

[0046] 3) Weigh 10 mg of digoxin and dissolve it to 10 mg / mL with DMF buffer to obtain a digoxin-DMF solution;

[0047] 4) Weigh 50mg of succinic anhydride and dissolve it to 5mg / mL with 10ml DMF to obtain a succinic anhydride-DMF solution;

[0048] 5) Weigh 20mg of lysine and dissolve it to 20mg / mL with 1mL of 0.01M NaHCO3 buffer with PH=9.0;

[0049] 6) Add 0.5 mL of digoxin-DMF solution to 10 mL of succinic anhydride-DMF solution, and react at 65°C for 6 hours;

[0050] 7) After the reaction is completed, add 1.2 mL of tributylamine and 0.65 mL of isobutyl chloroformate, and react at room temperature for 30 minutes to obtain a DMF solution of digoxigenin-succinic anhydride;

[0051] 8) Add 1 mL of lysine solution with a concentration of 20 mg / mL to the DMF solution of digoxin-succinic anhydride, an...

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Abstract

The invention is a kit for measuring digoxin content by magnetic particle chemiluminescence method and a detection method thereof, and provides a preparation method of digoxin antigen derivatives, which can realize the indirect linking of digoxin antigens with biotin or enzymes, Two detection systems of biotinylated digoxin antigen derivative + antibody enzyme-labeled reagent + calibrator and enzyme-labeled digoxin antigen derivative + biotinylated digoxin mouse monoclonal antibody + calibrator were constructed in the kit. It can be used for solid-phase coupling and luminescence-labeled digoxin derivatives. It is suitable for coupling digoxin antigens in different situations such as biotin, enzyme labels and even proteins, so that different companies can be more accurate when testing. Flexible selection, and can be realized only by preparing the intermediate of digoxin antigen derivative. The correlation between the measured value and the given value of different detection systems established by using this derivative is above 0.96, and the correlation between the two detection systems is 0.9585. The reaction time of the whole reaction system is short, radioactive pollution is avoided, the validity period of the reagent is extended, the sensitivity is high, the test result is accurate, and the experimental operation process is simplified.

Description

Technical field [0001] The invention relates to a detection kit, in particular to a kit for determining the content of digoxin by a magnetic particle chemiluminescence method and a detection method thereof. Background technique [0002] Digoxin is the first effective drug in the treatment of heart failure and rapid ventricular rate control, but the effective blood concentration is narrow. Based on the relevant content in the "2008 European Society of Cardiology Guidelines for the Diagnosis of Acute and Chronic Heart Diseases", recommended digoxin The therapeutic concentration is 0.6-1.2ng / ml. When the concentration of local high octane exceeds 1.2ng / ml, the risk of death increases. In addition, the therapeutic index is low, toxic and side effects are large, and individual differences are large. Conventional doses may also lead to poisoning or fail to reach effective doses, making it difficult to grasp the dose of digoxin in clinical practice. Therefore, the detection of serum di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/58G01N33/531G01N33/534
CPCG01N33/531G01N33/534G01N33/58G01N33/9453
Inventor 鲁衡刘振世
Owner TAIZHOU ZECEN BIOTECH CO LTD
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