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Extraction method and application of firefly chitosan

An extraction method, firefly technology, applied in application, additional food elements, animal feed, etc., can solve the problems of unrelated firefly chitosan extraction and utilization, etc., to avoid chitosan quality decline, inhibit bacterial activity, and high purity Effect

Inactive Publication Date: 2018-06-19
FOSHAN JUCHENG BIOCHEM TECH RES & DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] About the extraction and utilization of firefly, there are few related reports, and there is no information about the extraction and utilization of firefly chitosan

Method used

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  • Extraction method and application of firefly chitosan
  • Extraction method and application of firefly chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting chitosan from fireflies, comprising the steps of:

[0029] (1) Collect fireflies, clean them, scald them to death with hot water, remove the contents, dry them, and crush them through a 50-mesh sieve to obtain firefly powder;

[0030] (2) Take the firefly powder in step (1), add 0.8mol / L citric acid, the mass volume ratio (g / mL) of firefly powder to citric acid is 1:3, soak at a constant temperature at 48°C, and change it every 20 minutes Citric acid once, until no bubbles are generated, then washed to neutral, and dried to obtain decalcified firefly powder;

[0031] (3) Take decalcified firefly powder, add 0.9mol / L NaOH solution, the mass volume ratio (g / mL) of decalcified firefly powder to NaOH solution is 1:2, remove protein, wash with water until neutral, and obtain chitin ;

[0032] (4) adding 2 times the amount of potassium permanganate solution to the chitin in step (3) for decolorization to obtain decolorized chitin;

[0033] (5) Add 2...

Embodiment 2

[0035] A method for extracting chitosan from fireflies, comprising the steps of:

[0036] (1) Collect fireflies, clean them, scald them to death with hot water, remove the contents, dry them, and crush them through a 40-mesh sieve to obtain firefly powder;

[0037] (2) Take the firefly powder in step (1), add 0.6mol / L citric acid, the mass volume ratio (g / mL) of firefly powder to citric acid is 1:5, soak at a constant temperature at 45°C, and change it every 20 minutes Citric acid once, until no bubbles are generated, then washed to neutral, and dried to obtain decalcified firefly powder;

[0038] (3) Take decalcified firefly powder, add 0.8mol / L NaOH solution, the mass volume ratio (g / mL) of decalcified firefly powder to NaOH solution is 1:1, remove protein, wash with water until neutral, and obtain chitin ;

[0039] (4) adding 1 times the amount of potassium permanganate solution to the chitin in step (3) for decolorization to obtain decolorized chitin;

[0040] (5) Add 1...

Embodiment 3

[0042] A method for extracting chitosan from fireflies, comprising the steps of:

[0043] (1) Collect fireflies, clean them, scald them to death with hot water, remove the contents, dry them, and crush them through a 60-mesh sieve to obtain firefly powder;

[0044] (2) Take the firefly powder in step (1), add 1.0 mol / L citric acid, the mass volume ratio (g / mL) of firefly powder to citric acid is 1:1, soak at a constant temperature at 50°C, and change it every 20 minutes Citric acid once, until no bubbles are generated, then washed to neutral, and dried to obtain decalcified firefly powder;

[0045] (3) Take decalcified firefly powder, add 1.0mol / L NaOH solution, the mass volume ratio (g / mL) of decalcified firefly powder to NaOH solution is 1:3, remove protein, wash with water until neutral, and obtain chitin ;

[0046] (4) adding 3 times the amount of potassium permanganate solution to the chitin in step (3) for decolorization to obtain decolorized chitin;

[0047] (5) addi...

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PUM

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Abstract

The invention discloses an extraction method and application of firefly chitosan, and further provides a fodder. The extraction process of the firefly chitosan comprises the steps of cleaning and drying fresh fireflies, then decalcifying, removing protein and decoloring, and finally, extracting through an ultrasonic technology matched with the solvent under the aid of alkali, so as to obtain the chitosan. In the extraction process of the firefly chitosan, decalcification and deacetylation help reduce product impurities effectively, the ultrasonic technology cooperates with the solvent so as tofurther promote deacetylation, so that the product quality is higher, and the product can improve the immunity when being used in the fodder and can prohibit bacteria activity,.

Description

technical field [0001] The invention relates to a method for extracting chitosan, in particular to a method for extracting chitosan from fireflies. Background technique: [0002] Fireflies are insects of the Fluoridae family of Coleoptera. They are called fireflies because their tails can emit fluorescence. In order to protect itself, its body is flat and slender, its head is small, its body wall and elytra are soft, and its head is covered by a large pronotum cover, which is rich in chitosan. [0003] Chitosan, also known as deacetylated chitin, is obtained through deacetylation of chitin, which exists widely in nature, and has a wide range of uses. [0004] There are few related reports on the extraction and utilization of firefly chitosan, and there is no information on the extraction and utilization of firefly chitosan. Invention content: [0005] The object of the present invention is to provide a kind of method that extracts chitosan from firefly, to solve above-me...

Claims

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Application Information

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IPC IPC(8): C08B37/08A23K50/50A23K20/163
CPCC08B37/0003A23K20/163A23K50/50
Inventor 不公告发明人
Owner FOSHAN JUCHENG BIOCHEM TECH RES & DEV CO LTD
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