Preparation method of cliff chrysanthemum bud differentiation culture solution

Abstract

The invention discloses a preparation method of a cliff chrysanthemum bud differentiation culture solution. The preparation method comprises the following steps: preparing a culture solution and preparing a slow release culture solution, wherein the culture solution is prepared from the following components in parts by weight: 700-800 parts of an ethanol solution, 30-35 parts of sucrose, 15-16 parts of honey, 0.2-0.3 parts of vitamin B1, 2.5-3.5 parts of an active ingredient, 0.012-0.022 parts of active polypeptide and 0.5-0.6 parts of an auxin mixture. The culture solution is added to a vinylalcohol aqueous solution of glycerol, a reaction is performed at a certain temperature, and the cliff chrysanthemum bud differentiation culture solution is obtained. The method has the beneficial effects as follows: active polypeptide in the culture solution can effectively prevent auxin from being decomposed by photooxidation and losing the regulating function, the differentiation capacity of cliff chrysanthemum buds is improved, and flower buds can be distinguished from leaf buds gradually; the slow-release culture solution has a slow-release effect on active substances in the culture solution, so that the differentiation effect of the active substances in the culture solution on the cliff chrysanthemum buds is prolonged, and differentiation efficiency is improved.

Description

technical field [0001] The invention relates to the field of flower bud differentiation culture fluid, in particular to a preparation method of the cliff chrysanthemum bud differentiation culture fluid. technical background [0002] Cliff chrysanthemum is a pruned form of chrysanthemum. Usually choose single-petal type, many branches, thin and soft branches, and densely flowered small flower varieties, imitating the natural posture of wild chrysanthemums hanging in the mountains, and pruning into drooping cliffs. The key to cultivation is to use a bamboo frame to lure the trunk forward and pick the heart in time. The standard of appreciation is that the flowering branches hang upside down, the main trunk is on the middle line, the side branches are evenly distributed, the front is narrow and the back is wide, the flowers are plump, the flowering period is consistent, and the length wins. The basic form of Cliff Chrysanthemum is that the whole plant spreads obliquely or dro...

AI Technical Summary

Problems solved by technology

[0005] The Chinese invention patent of CN 102511290 B discloses a method for promoting the flower bud differentiation of osmanthus fragrans. The method of the invention sprays 500ppm of paclobutrazol or 250ppm of diethylhydrazide to promote the flower bud differentiation of osmanthus osmanthus when the spring shoots are basically capped, increasing the number of flower buds and The number of blooms prolongs the ornamental period and improves the overall ornamental quality, and has the characteristics of simple implementation, but the inventive method only uses paclobutrazol or diethylhydrazide to promote the differentiation of sweet-scented osmanthus flower buds, and its effect is not significant, and the drug effect time of paclobutrazol is too long, which affects young leaves. Growth, prone to small leaves and no plant growth