Screening method of feed probiotics capable of preventing chicken's infection caused by clostridium perfringens

Abstract

The invention discloses a screening method of feed probiotics capable of preventing chicken's infection caused by clostridium perfringens. The method comprises following steps: screening chickens infected by clostridium perfringens in clinic and disease resistant chickens; collecting the intestinal chyme samples; carrying out high throughput sequencing on microbial floras in the intestinal tractsof infected chickens and disease resistant chickens; comparing and analyzing the microbes in the intestinal tracts of infected chickens and disease resistant chickens through a metagenomics method; and screening and identifying probiotics capable of preventing infection caused by clostridium perfringens. According to the method, the metagenomics big data of floras in the intestinal tracts of infected chickens and disease resistant chickens is analyzed; probiotics capable of preventing infection caused by clostridium perfringens are selected from massive microbial floras in the intestinal tracts of disease resistant chickens, the screened probiotics are from same animals, the rejection between probiotics and a host is avoided, moreover, the screening is targeted, aimless screening is avoided, and a large amount of labor, resources, and time is saved.

Description

Technical field [0001] The present invention relates to the technical field of animal husbandry and veterinary medicine, in particular to a method for screening feed probiotics against infection by Clostridium perfringens in chickens. Background technique [0002] Clostridium perfringens is an important human and animal intestinal symbiotic bacteria and conditional pathogenic bacteria, which can produce a variety of exotoxins and is pathogenic to humans and many animals, especially for chicken necrotizing enteritis The outbreak is of great significance. The disease can not only reduce animal production performance and feed remuneration, increase mortality, and cause huge economic losses, but also cause human infection through chicken products, posing a major threat to human health. In the chicken industry, the addition of antibiotics is usually used to control chicken necrotizing enteritis. With the continuous application of antibacterial drugs, the number of resistant strains i...

AI Technical Summary

Problems solved by technology

Although a large number of probiotics have been screened in my country and widely used in production practice, these probiotics are mainly used to promote the digestion and absorption of nutrients in the intestinal tract of animals and promote growth, and many probiotics do not come from the body. Animals, after entering the body of the host, it is difficult to adapt to the host, and it is difficult to exert its exact effect

[0004] Traditional probiotic screening techniques mainly include in vitro antibacterial methods and in vivo bacterial attack methods, which have the disadvantages of blindness, low efficiency, poor pertinence, and mostly exogenous bacteria.

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