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Composite biological preservative fresh keeping agent based on epsilon-polylysine-containing fermentation liquid

A technology of polylysine and bio-preservation, applied in fermentation, fruit and vegetable preservation, food preservation, etc., to reduce costs, improve anti-corrosion effect, and expand the effect of antibacterial spectrum

Pending Publication Date: 2018-06-15
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the direct production of biological preservatives with microbial fermentation broth

Method used

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  • Composite biological preservative fresh keeping agent based on epsilon-polylysine-containing fermentation liquid
  • Composite biological preservative fresh keeping agent based on epsilon-polylysine-containing fermentation liquid
  • Composite biological preservative fresh keeping agent based on epsilon-polylysine-containing fermentation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Fermentation of Streptomyces ahygroscopic Str-8

[0022] Incline medium (g / L): soluble starch 20, KNO 3 1, NaCl 0.5, K 2 HPO 4 0.5, MgSO 4 0.5, FeSO 4 0.01, agar 20, the solvent is water, mix evenly, adjust the pH to 7.1-7.5, and sterilize at 121°C for 20 minutes.

[0023] Fermentation medium (g / L):

[0024] Yeast extract 5.0g, glucose 20g, (NH 4 ) 2 SO 4 7.0g, K 2 HPO 4 0.8g, KH 2 PO 4 1.36g, MgSO 4 ·7H 2 O 0.5g, ZnSO 4 ·7H 2 O 0.04g, FeSO 4 ·7H 2 O 0.3g, the balance is water. According to the above composition and content, mix the raw materials evenly, and sterilize at 121°C for 20 minutes.

[0025] Inoculate Streptomyces nonhygroscopicus Str-8 on the slant medium, culture at 28-30°C for 72 hours, then transfer a ring of spores of this fungus to a 250ml Erlenmeyer flask containing 50ml of fermentation medium, culture at 30°C for 72 hours, shake the flask The rotation speed is 150r / min, and the seed liquid is obtained.

[0026] Take 150mL ...

Embodiment 2

[0045] Get the ε-polylysine 30.0g / L microbial fermentation supernatant M 0.5L containing ε-polylysine 30.0g / L of embodiment 1, add and get distilled water 29.5L, prepare ε-polylysine 0.05% (W / V g / ml) containing Mixed solution 30L; Take by weighing 150 grams (0.5%, W / V) L-malic acid and add in the mixed solution, after stirring to fully dissolve, then add 15 grams (0.05%, W / V) Natamycin and stir to dissolve, then Add 15 grams (0.05%, W / V) of L-alanine, and prepare 30L composite biological preservative A after dissolving.

[0046] Control 1: 30 L of distilled water was taken, and 15 grams of ε-polylysine was added to prepare 30 L of a mixed solution containing 0.05% (W / V) of ε-polylysine. Take by weighing 150 grams (0.5%, W / V) L-malic acid and add in the mixed solution, after stirring and fully dissolving, then add 15 grams (0.05%, W / V) Natamycin stirring and dissolving, then add 15 grams ( 0.05%, W / V) L-alanine, after dissolving, be prepared into 30L composite biological prese...

Embodiment 3

[0057] Get 2.0L of the microbial fermentation supernatant M containing ε-polylysine 30.0g / L in Example 1, add 28L of distilled water to prepare 30L of mixed solution containing ε-polylysine 0.2% (W / V); Take by weighing 300 grams (1.0%, W / V) L-malic acid and add in the mixed solution, after stirring and fully dissolving, then add 60 grams (0.2%, W / V) Natamycin and stir to dissolve, then add 30 grams ( 0.1%, W / V) L-alanine, after dissolving, be prepared into 30L composite biological preservative A.

[0058] Control 1: Take 30 L of distilled water and add 60 grams of ε-polylysine to prepare 30 L of mixed solution containing 0.2% (W / V) of ε-polylysine. Take by weighing 300 grams (1.0%, W / V) L-malic acid and add in the mixed solution, after stirring and fully dissolving, then add 60 grams (0.2%, W / V) Natamycin and stir to dissolve, then add 30 grams ( 0.1%, W / V) L-alanine, after dissolving, be prepared into 30L composite biological preservative B.

[0059] Control 2: 28 L of dist...

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Abstract

The invention discloses a composite biological preservative fresh keeping agent based on epsilon-polylysine-containing fermentation liquid. Each 100ml of the composite biological preservative fresh keeping agent comprises an epsilon-polylysine-containing microbial fermentation supernatant with the addition quantity meeting the following requirements that the content of epsilon-polylysine is 0.05-0.3g, the content of L-malic acid is 0.5-2g, the cotnent of L-lactamic acid is 0.05-0.5g, the content of natamycin is 0.05-0.3g and the balance of water. According to the composite biological preservative fresh keeping agent based on epsilon-polylysine-containing fermentation liquid, the epsilon-polylysine-containing microbial fermentation supernatant is directly used for preparation, and a complextechnology for separation and extraction of a biological preservative namely epsilon-polylysine is omitted, so that the cost of the biological preservative is greatly reduced. The method is simple and easy to operate. Experiment proves that the made composite biological fresh keeping agent is better in bacterial effect than a contrast group of directly adding pure epsilon-polylysine.

Description

Technical field: [0001] The invention belongs to the technical field of antiseptic and fresh-keeping of fruits, and in particular relates to a composite biological anti-corrosion and fresh-keeping agent based on a fermentation liquid containing ε-polylysine. Background technique: [0002] Although fruit is more and more popular and paid attention to because of its high nutritional value and health-care functions, the fruit is extremely perishable, and microorganisms are the most important cause of fruit rot. The safety issue of fruit preservation and preservation has not received enough attention. [0003] At present, the preservatives used in various fruits are mainly chemical fungicides. Due to the harm of chemical fungicides to the human body, the United States, Japan and other countries prohibit the use of chemical fungicides on post-harvest fruits. Although chemical antiseptic preservatives have good antiseptic and fresh-keeping effects, most of them have different deg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154A23B7/155C12P13/02
CPCA23B7/154A23B7/155C12P13/02
Inventor 柏建玲王惠惠莫树平张菊梅吴清平吴军林何莹龙丘明泉陈谋通
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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