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A kind of preparation method of Aspergillus niger provenance spore agent

A spore inoculum and Aspergillus niger technology are applied in the field of preparation of provenance spore inoculum in the process of making koji, can solve the problems of quality fluctuation of bran koji, large production volume of eggplant bottles, complicated inoculation operation and the like, and achieve the inoculation link. The effect of simplifying, reducing the chance of contamination, and reducing the chance of contamination

Active Publication Date: 2021-07-06
JIANGSU GUOXIN UNION ENERGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this inoculation method is that the production of eggplant bottles is relatively large, the inoculation operation is cumbersome, and the number and quality of spores scraped from each eggplant bottle will have certain differences, which will cause certain fluctuations in the quality of bran.

Method used

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Examples

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Effect test

Embodiment 1

[0031] A kind of preparation method of aspergillus niger provenance spore agent, described method comprises the steps:

[0032] (1) Cultivate provenance spores in corncob culture medium;

[0033] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 800g of corn steep liquor powder and glucose, 120g of ammonium sulfate, mix and dissolve with water, and make up to 6L after dissolution. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0034] Take two 2000ml triangular flasks, fill each bottle with 80g of corncob medium, add 128ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization, pick a ring of spores with an inoculation needle, and place ...

Embodiment 2

[0041] A kind of preparation method of aspergillus niger provenance spore agent, described method comprises the steps:

[0042] (1) Cultivate provenance spores in corncob culture medium;

[0043] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: Take 200g of corn steep liquor powder and glucose, 60g of ammonium sulfate, 3.4L of soybean meal hydrolyzate, mix and dissolve with water, and make up to 6L after dissolving. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0044] Take two 2000ml triangular flasks, fill each bottle with 100g of corncob medium, add 120ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization, pick a ring of spores with ...

Embodiment 3

[0051] A kind of preparation method of aspergillus niger provenance spore agent, described method comprises the steps:

[0052] (1) Cultivate provenance spores in corncob culture medium;

[0053] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 400g of corn steep liquor powder and glucose, 120g of ammonium sulfate, 1L of soybean meal hydrolyzate, mix and dissolve with water, and make up to 6L after dissolving. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0054] Take two 2000ml triangular flasks, fill each bottle with 66g of corn cob medium, add 100ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization, pick a ring of spores with a...

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Abstract

The invention discloses a method for preparing an Aspergillus niger provenance spore agent, the method comprising the following steps: (1) cultivating provenance spores in a corn cob medium; (2) preparing provenance spores by adopting a liquid wax separation method (3) the provenance spore bacterial agent prepared in step (2) is inserted into a bran koji culture container and cultivated to make koji. The invention solves the limitations and instability of the eggplant bottle provenance, and greatly reduces the production amount of the provenance, reduces the operation steps of inoculation in the koji making process, avoids the occurrence of bacterial contamination, and greatly saves the production cost of the enterprise. The labor cost has created better economic benefits for the enterprise.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a preparation method of provenance spore bacterial agent in the process of Aspergillus niger koji making. Background technique [0002] The preparation of bran koji is the basis of citric acid production. At present, there are two main koji-making methods for citric acid production. One is to use bran to cultivate in a glass conical flask. The method is to wash the bran with water to remove the impurities in the bran. Flour, remove part of the water in the bran, so that the bran contains about 50% water, loosely pack it into a 2000ml triangular bottle, about 35 grams per bottle, put it into a ring eggplant bottle after sterilization, and cultivate Aspergillus niger spores 7 -10 days, each eggplant bottle can be connected with 50-60 triangular flasks, and it will be used after being cultivated and matured and passing the sampling inspection. When in use, about 150 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/685
CPCC12N1/14C12N3/00
Inventor 高金宝刘振强孙士健裴云朱剑飞李小晔胡志杰
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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