Method for extracting total dried orange peel flavonoid from dried orange peel by ultrasonic-assisted method and application of total dried orange peel flavonoid to cosmetics
An ultrasonic and cosmetic technology, applied in the field of comprehensive utilization of tangerine peel, can solve problems such as undiscovered targeted research, discarded citrus seeds, waste of resources, etc., achieve excellent sun protection ability and stability, high extraction efficiency, and low energy consumption Effect
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Embodiment 1
[0025] This example examines the relationship between the extractant and the absorbance of the tangerine peel flavone extract.
[0026] Accurately weigh 4 parts of tangerine peel samples of 80-100 mesh, divide them into two groups, the first group is two parts of 20mg tangerine peel, the second group is two parts of 90mg tangerine peel, add 1,2-propanediol in the first group and the second group respectively 20mL each of two different solvents and absolute ethanol, after infiltration, ultrasonically extract at 30°C for 20min and filter, the filtrate is centrifuged at 4000r / min for 15min, take 2ml of supernatant, and measure its ultraviolet absorption spectrum between 200-400nm, And calculate the average absorbance value of its UVC, UVB and UVA.
[0027] Taking the extract of tangerine peel flavonoids on the ultraviolet spectrum absorbance as the evaluation standard, the influence of the solvent type on the ultrasonic extraction process is shown in figure 1 and Table 1. Depen...
Embodiment 2
[0031] This example examines the relationship between the solid-liquid ratio of tangerine peel and organic solution extractant and the absorbance of tangerine peel flavone extract.
[0032] Accurately weigh 8 samples of tangerine peel of 80-100 mesh, each of which is 90mg, 110mg, 150mg, 220mg, 280mg, 320mg, 380mg, 390mg, add 20mL of 1,2-propanediol respectively, after infiltration, ultrasonically extract at 30°C for 20min and filter. The filtrate was centrifuged at 4000r / min for 15min, and 2ml of the supernatant was taken, and the ultraviolet absorption spectrometry was the same as in Example 1.
[0033] Taking the extract of tangerine peel flavonoids on the ultraviolet spectrum absorbance as the evaluation standard, the effect of the solid-liquid ratio of tangerine peel and organic solution extractant on the sunscreen activity of tangerine peel flavonoids extract is as follows: figure 2 shown. Depend on figure 2 It can be seen that with the increase of the solid-liquid ra...
Embodiment 3
[0035] This example examines the relationship between ultrasonic extraction time and the absorbance of tangerine peel flavonoids extract.
[0036] Accurately weigh 4 parts of tangerine peel samples of 80-100 mesh, each part is 320mg, add 20mL of 1,2-propanediol respectively, after infiltration, ultrasonically extract at 30°C, the extraction time is 20min, 40min, 60min, 80min, 100min, 120min and filter , the filtrate was centrifuged at 4000r / min for 15min, and 2ml of the supernatant was taken, and the ultraviolet absorption spectrometry was the same as in Example 1.
[0037] Taking the ultraviolet spectrum absorbance of the tangerine peel flavonoid extract as the evaluation standard, the influence of ultrasonic extraction time on the sunscreen activity of the tangerine peel flavonoid extract is shown in Table 2. It can be seen from Table 2 that when the extraction time is 20-80 min, the sunscreen activity of the tangerine peel extract increases with the prolongation of the extr...
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