Purification method of low-molecular-weight sargassum fusiforme polysaccharides

Abstract

The invention discloses a purification method of low-molecular-weight sargassum fusiforme polysaccharides. The purification method comprises the following steps of (1): ultrafiltration classification:enabling an argassum fusiforme polysaccharide solution containing different molecular weights to pass through ultrafiltration systems, the molecular weight cutoffs of which are 10kDa, 5kDa and 1kDa respectively and which are further sequentially connected in series, and enabling primary tail liquid of the 10kDa ultrafiltration system to flow into a circulation cup of the 5kDa ultrafiltration system to carry out water circulation ultrafiltration, wherein the weight-average molecular weight of the primary tail liquid is smaller than and equal to 10kDa; enabling secondary tail liquid of the 5kDaultrafiltration system to flow into a circulation cup of the 1kDa ultrafiltration system to carry out the water circulation ultrafiltration until tertiary tail liquid of the 1kDa ultrafiltration system does not contain polysaccharide, wherein the weight-average molecular weight of the secondary tail liquid is smaller than and equal to 5kDa; afterwards, rinsing the polysaccharide solution in pipelines of the ultrafiltration systems by using water and collecting respectively; (2), concentration and freeze-drying: collecting the polysaccharide solution of each interval section, carrying out reduced pressure concentration, and then carrying out reduced pressure freezing-drying, so that the low-molecular-weight sargassum fusiforme polysaccharides of intervals of the corresponding molecular weights can be obtained.

Description

technical field [0001] The invention belongs to the fields of biochemistry and separation and purification, and specifically refers to a method for purifying low-molecular-weight hijiki polysaccharides. Background technique [0002] Hijiki polysaccharide is one of the active ingredients of hijiki. Studies have shown that hijiki polysaccharides not only have anti-tumor, anti-virus, anti-coagulation, hypolipidemic, and hypoglycemic activities, but also can improve the body's immunity, and have the effects of scavenging free radicals, anti-oxidation, and anti-aging. Therefore it has high medicinal value. [0003] In the prior art, the separation and purification of hijiki polysaccharides generally need to go through extraction, decolorization, deproteinization and other steps. The traditional polysaccharide purification method is cumbersome, time-consuming, large loss, and the amount of sample that can be purified in one operation is small, which is very easy. Affect polysacc...

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Problems solved by technology

[0003] In the prior art, the separation and purification of hijiki polysaccharides generally need to go through extraction, decolorization, deproteinization and other steps. The traditional polysaccharide purification method is cumbersome, time-consuming, large loss, and the amount of sample that can be purified in one operation is small, which is very easy. Affect polysaccharide quality and yield

[0004] In the prior art, the purification and refining of low-molecular-weight hijiki polysaccharides has not been reported. If the traditional polysaccharide purification method is used, the cost is high, the process is complicated, the product yield is very low, and the purity is generally not high. The obtained polysaccharide has a large molecular weight range and molecular weight. The inhomogeneous composition finally leads to the inability to realize the industrial preparation of hijiki polysaccharides with good uniformity, which is also a major problem that plagues the development of hijiki polysaccharides

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