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Compound in xanthoceras sorbifolia shell, and preparation method and application thereof

A technology of compounds and compound structural formulas, applied in the field of medicine, can solve the problem of AD without specific drugs and other problems

Active Publication Date: 2018-06-01
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now that our country is entering an aging society, it is expected that the number of AD patients in our country will continue to increase, but there is no specific drug for AD at present, so it is necessary to extract and separate the sorbetia sorbifolium husk, and find that the active pentacyclic Triterpenoids provide a material basis for the further development of drugs with anti-AD activity

Method used

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  • Compound in xanthoceras sorbifolia shell, and preparation method and application thereof
  • Compound in xanthoceras sorbifolia shell, and preparation method and application thereof
  • Compound in xanthoceras sorbifolia shell, and preparation method and application thereof

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Effect test

Embodiment 1

[0035] The method for extracting and separating new compound in the Chinese crown fruit shell of the present invention, concrete steps are as follows:

[0036] Get 9.5 kg of sorbifolia husks, pulverize, sieve, and extract 3 times with 70% ethanol under reflux, each time for 2 hours, and the amount of each extraction solvent is 8, 6, and 4 times of the amount of medicinal materials respectively; the combined filtrate, The solvent is recovered under reduced pressure, evaporated to dryness to obtain the extract of Xantho sorbifolium husk; the extract is dispersed in deionized water, prepared as a sample solution for macroporous adsorption resin column chromatography, and loaded on the D101 type macroporous adsorption resin column chromatography, Absorbed overnight; the next day, gradient elution was performed with pure water, 50% ethanol, 70% ethanol, and 95% ethanol to obtain several fractions, and the solvent was recovered under reduced pressure, and the extract of each fraction...

Embodiment 2

[0039] 3-O-β- D -glucopyranosyl(1→6)-(2’-angeloyl)-β- D -glucopyranosyl-28-O-β- D -glucopyranosyl(1→6)[α- L -rhamnopyranosyl(1→2)-β- D Structural identification of -glucopyranosyl]-21-O-acetyl-16-deoxybarringtoenol C

[0040] The compound is white powder (methanol), mp 220~230°C; (c 0.065MEOH); Liebermann-Burchard reaction was positive, and 10% concentrated sulfuric acid-ethanol solution was purple, suggesting that the compound might be a triterpenoid saponin compound; HR-ESI-MS gave m / z 719.3356[M+2Na ] + (calcd for 719.3355), IR(KBr)V max 3425, 2922, 1647, 1631, 1401, 1269, 1008cm -1 ; Determine that the molecular formula of this compound is C in conjunction with NMR spectrum 67 h 108 o 30 .

[0041] 1 H-NMR spectrum gives 7 characteristic angle methyl signals in the high field region, δ0.89(3H,s),0.97(3H,s),1.00(3H,s),1.03(3H,s), 1.06(3H, s), 1.15(3H, s), 1.19(3H, s); the signal of 12-position hydrogen proton δ5.48(1H, brs), suggesting that it may be an oleanan...

Embodiment 3

[0048] Compounds of the present invention are anti-Aβ 25-35 Protective effect of induction of PC12 cell injury

[0049] 1. Cell Culture

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Abstract

The invention belongs to the field of medicines, and relates to an application of a novel compound, which has effects of preventing and treating Alzheimer's disease and is extracted and separated fromdried shells of a plant xanthoceras sorbifolia, which belongs to the Xanthoceras Sapindaceae. In the invention, the novel compound is extracted and separated mainly through a traditional extraction and separation methods with combination of modern instrumental analytic methods, wherein the method mainly includes heating reflux extraction, macroporous adsorption resin column chromatography, silicagel column chromatography, ODS opened column chromatography, and preparative HPLC separation. A pharmacological experiment proves that the compound has excellent nervous protection effect on PC12 cell damage induced by A[beta]25-35; the compound can be used for further development and application of an anti-Alzheimer's disease medicine.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a compound extracted and separated from the sorbetia sorbifolium husk, a preparation method and application thereof. Background technique [0002] Xanthoceras sorbifolia Bunge, also known as papaya, mountain papaya, Sengdengmudao, Wendengge, belongs to the genus Xanthoceras of Sapindaceae (Sapindaceae). This oil plant has the title of "Northern Camellia". The whole body of sorbifolium sorbifolium can be used as medicine, and the dry xylem of its stems and branches are commonly used medicinal materials of the Mongolian people. The name of the Mongolian medicine is "Sira Sendeng". Sweet in taste, slightly bitter, cool in nature, can dispel wind and relieve pain, and restrain yellow water. It is often used to treat diseases such as gout and rheumatism. Xantho sorbifolium seed kernels are commonly used in the folks to treat enuresis in children. In modern times, they were develope...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P25/28
CPCC07H1/08C07H15/256C07J63/008
Inventor 毕开顺李清丁科文毕文川刘然许华容
Owner SHENYANG PHARMA UNIVERSITY
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