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Preparation method of thermosensitive hydrogel with adhesiveness and antibacterial activity

A temperature-sensitive hydrogel and bacteriostatic technology, applied in the field of medical materials, can solve problems such as hydrogels are easy to adhere to bacteria and unfavorable for wound healing

Inactive Publication Date: 2018-06-01
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hydrogels with solid adhesive properties are easy to adhere to bacteria, which is not conducive to wound healing

Method used

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  • Preparation method of thermosensitive hydrogel with adhesiveness and antibacterial activity
  • Preparation method of thermosensitive hydrogel with adhesiveness and antibacterial activity
  • Preparation method of thermosensitive hydrogel with adhesiveness and antibacterial activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. The preparation of HBC adopts the existing technology (refer to patent CN201110214776.X), and the steps are as follows:

[0016] First take a certain amount of chitosan powder and disperse it in 50% NaOH aqueous solution for alkalization treatment, the amount of NaOH aqueous solution is 10mL per gram of chitosan, stir under N2 protection for 24h, squeeze dry to remove the liquid, and obtain a solid; then Add the solid matter into isopropanol aqueous solution, the amount of isopropanol aqueous solution is 20mL per gram of chitosan, then add a certain volume of 1,2-epoxybutane, react for 1 hour, and use microwave power of 600W-1200W , raise to a certain reaction temperature, continue to heat and stir for a certain period of time to react, stop the microwave to end the reaction, cool to the temperature before the temperature rise; finally adjust the pH of the system to neutral, filter out the insoluble matter, add ethanol, centrifuge, and vacuum dry. get HBC;

[0017] ...

Embodiment 2

[0020] 1. The preparation of HBC is the same as step 1 in Example 1;

[0021] 2. Dissolve 15mg EPL in 1mL deionized water to obtain EPL solution, add 4.60mg NHS and 7.67mgEDC to the solution, and activate the reaction under dark conditions; dissolve 19.72mg L-DOPA in 10mL deionized water to obtain L-DOPA solution , add 23.02mg NHS and 38.34mg EDC to the solution to activate the carboxyl group; add the activated EPL solution and L-DOPA solution to 5mL HBC solution with a concentration (w / v) of 1%, and stir overnight with magnetic force; the reaction ends After dialysis, freeze-drying to obtain eLHBC;

[0022] 3. eLHBC was dissolved in deionized water at low temperature with a concentration (w / v) of 4%, and a hydrogel was obtained after heating.

Embodiment 3

[0024] 1. The preparation of HBC is the same as step 1 in Example 1;

[0025] 2. Dissolve 15mg EPL in 1mL deionized water to obtain EPL solution, add 4.60mg NHS and 7.67mgEDC to the solution, and activate the reaction under dark conditions; dissolve 39.44mg L-DOPA in 20mL deionized water to obtain L-DOPA solution , add 46.04mg NHS and 76.68mg EDC to the solution to activate the carboxyl group; add the activated EPL solution and L-DOPA solution to 5mL HBC solution with a concentration (w / v) of 1%, and stir overnight with magnetic force; the reaction ends After dialysis, freeze-drying to obtain eLHBC;

[0026] 3. eLHBC was dissolved in deionized water at low temperature with a concentration (w / v) of 4%, and a hydrogel was obtained after heating.

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Abstract

The invention discloses a preparation method of thermosensitive hydrogel with adhesiveness and antibacterial activity. Firstly, natural chitosan is subjected to hydroxybutylation modification to havethermosensitivity (patent number: 201110214776.X); secondly, HBC (hydroxybutyl chitosan) is grafted with L-DOPA (levodopa) with the chemical name of 3-hydroxy-L-tyrosine, and L-DOPA contains catechingroups, so that the hydrogel has good adhesiveness; then, HBC is grafted with EPL (epsilon-poly-L-lysine), EPL is a polypeptide and has the antibacterial efficacy by use of protonated amino groups. The composite gel material has adhesiveness and antibacterial activity and possibly has wide application prospects in the aspects of tissue engineering and skin repairing.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a method for preparing a temperature-sensitive hydrogel with adhesion and antibacterial properties. Background technique [0002] The skin is the largest organ of the human body, covering the whole body, and protecting various tissues and organs in the body from physical damage, chemical damage and invasion of pathogenic microorganisms. Large areas of skin are exposed to the external environment and are easily damaged. Traditional skin wound dressings include gauze, bandages, etc., but all have certain limitations and cannot fully meet the needs of chronic wound healing. The ideal skin repair product itself should have a certain degree of adhesion, antibacterial and biocompatibility, and will not cause harm to the body. Therefore, it is of great significance to develop effective skin repair products. [0003] As a natural cationic polymer, chitosan has at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/18A61L27/50A61L27/52A61L27/54A61L27/60C08J3/075
CPCA61L27/18A61L27/20A61L27/50A61L27/52A61L27/54A61L27/60A61L2300/404C08J3/075C08J2377/04C08J2405/08C08L5/08C08L77/04
Inventor 刘雅田美平陈西广程晓杰孔明冯超
Owner OCEAN UNIV OF CHINA
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