Identification of toxoplasma gondii chronic infection male mouse genital system target gene and clinical application thereof

Abstract

The invention provides a method for applying an RNA-seq technology to research toxoplasma gondii chronic infection mouse epididymis tissue differential expression genes. The method comprises the following steps of (1) extracting RNA of mouse epididymis tissues; (2) carrying out library construction and sequencing for the RNA obtained in the step (1); (3) carrying out bioinformatics analysis on a sequencing result of the step (2), obtaining the toxoplasma gondii chronic infection mouse epididymis tissue differential expression genes, and screening and identifying reproduction-related genes. Themethod for the toxoplasma gondii chronic infection mouse epididymis tissue differential expression genes provided by the invention can be used for quickly and effectively researching a gene expression difference, and identifying whether an epididymis is infected with toxoplasma gondii or not through using a qPCR method for detecting a screened target gene, has an important theoretical value and an application prospect on improving reproductive potential of male animals and animal reproduction rate, and is beneficial to quickly positioning detection targets and prevention and cure drug targetswith research values or commercial application values by adopting the genes as molecular markers for identifying whether reproductive systems of the male animals are infected with the toxoplasma gondii or not.

Description

Technical field [0001] The present invention relates to the field of parasitology, and relates to a study on the mechanism of Toxoplasma gondii infection in the epididymal tissue of male mice; in particular, it relates to a method for obtaining differentially expressed genes in the epididymis tissue of mice chronically infected with Toxoplasma by using RNA-seq technology, revealing Toxoplasma gondii The expression regulation mechanism of worms on the epididymis of the male reproductive system, the acquisition of the main genes for specific disease signals, and a detection method for Toxoplasma gondii infection. Background technique [0002] Toxoplasma gondii is an intracellular parasitic protozoan that can infect almost all warm-blooded animals. After a pregnant woman is infected with Toxoplasma gondii, the vertical transmission of the parasite to the fetus can cause fetal malformations, miscarriage and death. Toxoplasma infection can also cause serious consequences such as dama...

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Problems solved by technology

[0004] At present, most of the researches on male infertility caused by Toxoplasma gondii infection focus on the epidemiological aspect. Toxoplasma gondii antibodies were detected by serological methods, Toxoplasma gondii DNA was detected in semen by PCR method, and Toxoplasma gondii was observed in pathological tissue sections. Infection causes tissue and cellular damage, but there is no report on the application of transcriptome sequencing technology to study the differentially expressed genes in epididymis tissues in the male reproductive system of mice chronically infected with Toxoplasma gondii

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