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Kit and detection method for detecting salmonellae by combining recombinase isothermal amplification and immune colloidal gold test strip

A technology of colloidal gold test paper and constant temperature amplification, applied in the direction of microorganism-based methods, biochemical equipment and methods, measuring devices, etc., can solve problems such as patent publications that have not been found, and achieve intuitive and accurate reaction results, sensitive detection, and consumption The effect of duration

Inactive Publication Date: 2018-05-22
中科智测(天津)科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through the search, no patent publications related to the application of the present invention have been found

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  • Kit and detection method for detecting salmonellae by combining recombinase isothermal amplification and immune colloidal gold test strip
  • Kit and detection method for detecting salmonellae by combining recombinase isothermal amplification and immune colloidal gold test strip
  • Kit and detection method for detecting salmonellae by combining recombinase isothermal amplification and immune colloidal gold test strip

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Embodiment Construction

[0044] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0045] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0046] A recombinase constant temperature amplification combined with immunocolloidal gold test strip detection kit for Salmonella, is characterized in that: said kit includes Salmonella gene primer set DNA, recombinase polymerase constant temperature amplification kit (TwistAmpBasic), sterile Distilled water and nucleic acid immune colloidal gold test strips:

[0047] Wherein, the Salmonella gene primer set DNA is a forward primer and a reverse primer for constant temperature amplification of Salmonella, the forwar...

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Abstract

The invention discloses a kit for detecting salmonellae by combining recombinase isothermal amplification and an immune colloidal gold test strip. The kit comprises a salmonella gene primer group DNA,a recombinase isothermal amplification skit, sterile distilled water and a nucleic acid immune colloidal gold test strip. The kit is convenient to operate and quite applicable to field detection; thedetection cost is low, and only 20min is required by the whole detection process; the sensitivity is 104 times higher than a limit of detection implemented by combining PCR (polymerase chain reaction) and agarose gel electrophoresis, so that the kit can be widely used for detecting various pathogenic bacteria, and the salmonellae can be detected accurately, sensitively and rapidly.

Description

technical field [0001] The invention belongs to the technical field of food detection, and relates to the detection of Salmonella in food, in particular to a kit for detecting Salmonella using a recombinant enzyme polymerase constant temperature amplification combined with an immune colloidal gold test strip and a method for detecting Salmonella using the kit. Background technique [0002] In recent years, due to the frequent occurrence of food safety incidents, the World Health Organization and consumers have paid more and more attention to food safety issues. Salmonella is a Gram-negative bacillus that is widely distributed in nature and can easily contaminate many foods such as meat, seafood, eggs, etc. Consumers who eat food contaminated by Salmonella will cause food poisoning. In my country, among the food poisoning caused by bacteria every year, the food poisoning caused by Salmonella ranks first, accounting for about 40%. The methods for detection and identification ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6844C12Q1/10G01N33/569C12R1/42
CPCC12Q1/6844G01N33/56916G01N2333/255C12Q2521/507
Inventor 任士常张志红
Owner 中科智测(天津)科技有限公司
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