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Culture medium for breed conservation of salted bruchus rufimanus and breed conservation method thereof

A culture medium and broad bean technology, applied in fish farming, climate change adaptation, animal feed, etc., can solve the problems of increasing the work of experimenters, failing to make, and increasing the risk of species conservation

Active Publication Date: 2018-05-22
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even in the context of hatchery requirements, no substantial effort has been made to cultivate this cilium as a food source
[0004] As an emerging biological bait, broad bean worms are greatly affected by seasonal changes in nature. Generally, salina is used as food in laboratory cultivation, but if salina is used for species preservation, experimenters also need to carry out the cultivation of salina. This not only increases the work of the experimenter but also increases the risk of conservation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Configure medium:

[0024] 100g of NaCl, 0.8g of KCl, 2.6g of MgCl 2 6H 2 O, 1.9 g MgSO 4 7H 2 O, 0.9 g CaSO 4 2H 2 O and 28mg of Angel dry yeast were dissolved in water to prepare 1L of culture medium.

[0025] Conservation:

[0026] Step 1: Add the prepared 140ml culture medium into a 250ml Erlenmeyer flask, use a pipette to draw 10ml of the mother liquor of broad bean worm salt into the Erlenmeyer flask where the culture medium is added, and plug the end of the Erlenmeyer flask with the prepared cotton plug mouth;

[0027] Step 2: Place the Erlenmeyer flask containing the culture medium and the mother liquor of Vicia saline in step 1 in a constant temperature incubator at a temperature of 20°C, and cultivate it with a light intensity of 1000 lux, and the white-to-black ratio of the light cycle is 1:1;

[0028] Step 3: Every 15 days, draw 10ml of the culture solution in the Erlenmeyer flask of Step 2 and transfer it to a new Erlenmeyer flask containing 140ml...

Embodiment 2

[0033] Configure medium:

[0034] 110g of NaCl, 0.9g of KCl, 2.5g of MgCl 2 6H 2 O, 2.0 g MgSO 4 7H 2 O, 0.8g CaSO 4 2H 2 O and 30mg of Angel dry yeast were dissolved in water to prepare 1L of culture medium.

[0035] Conservation:

[0036] Step 1: Add the prepared 140ml culture medium into a 250ml Erlenmeyer flask, use a pipette to draw 10ml of the mother liquor of broad bean worm salt into the Erlenmeyer flask where the culture medium is added, and plug the end of the Erlenmeyer flask with the prepared cotton plug mouth;

[0037] Step 2: Place the Erlenmeyer flask containing the culture medium and the mother liquor of Vicia saline in step 1 in a constant temperature incubator at a temperature of 20°C, and cultivate it with a light intensity of 1000 lux, and the white-to-black ratio of the light cycle is 1:1;

[0038] Step 3: Every 15 days, draw 10ml of the culture solution in the Erlenmeyer flask of Step 2 and transfer it to a new Erlenmeyer flask containing 140ml ...

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PUM

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Abstract

The invention provides a culture medium for breed conservation of salted bruchus rufimanus and a breed conservation method thereof. The culture medium comprises the following components in concentration: 95-115g / L of NaCl, 0.5-1.0g / L of KCl, 2.0-3.0g / L of MgCl26H2O, 1.5-2.5g / L MgSO47H2O, 0.5-1.5g / L CaSO42H2O and 26-30mg / L of Angel dried yeast, and the solvent is water. The invention aims at providing the culture medium for breed conservation of the salted bruchus rufimanus and the breed conservation method thereof, the breed conservation mode is simple, long-term breed conservation of the salted bruchus rufimanus is realized, and essential technical support is provided for in-depth research on the value of the salted bruchus rufimanus as live food and for basic theoretical research on other biological characteristics and the like.

Description

technical field [0001] The invention belongs to the field of salt broad bean worm, and in particular relates to a culture medium and a seed conservation method for salt broad bean worm. Background technique [0002] The broad bean worm belongs to the genus Vicia faba of the order Heterochaetes. It is a species with a higher taxonomic status among the planktonic ciliates and a larger species. The length of the living body is about 180 to 300 μm, and the width is about 67 to 125 μm. It is a relatively common species in the Yellow and Bohai Sea regions. Can form dominant species in closed high salinity water bodies. The importance of the saltworm as a potential live bait for juvenile fish was first suggested by Morris. Studies by Rhodes et al. have shown that the salt broad bean worm can replace rotifers as the opening bait for red snapper fry, and the growth effect of red snapper fry will be better. Biochemical composition and fatty acid profile analysis also indicated that ...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K20/24A23K10/18A01K61/20
CPCA23K10/18A23K20/24A23K50/80A01K61/20Y02A40/818
Inventor 齐红莉汤荣成徐海龙李蒿王茜李雪菡何建武郑晓楠
Owner TIANJIN AGRICULTURE COLLEGE
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