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Bacillus siamensis strain and application thereof

A technology of Bacillus and strains, applied in Bacillus siamese strains and its application fields, can solve the problems of undiscovered high-resistant varieties, etc., and achieve the effects of good application prospects, wide action spectrum, and large spore production

Active Publication Date: 2018-05-15
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the strong saprophytic ability and wide range of hosts of sheath blight, no high-resistance varieties have been found at home and abroad.

Method used

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  • Bacillus siamensis strain and application thereof
  • Bacillus siamensis strain and application thereof
  • Bacillus siamensis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Primary screening of strains

[0039] Collect 10 fresh feces samples of healthy piglets from all over Jilin Province, weigh 10g samples respectively, add 90mL sterile saline to prepare a sample suspension, and take 100μL 10 -2 、10 -3 、10 -4The diluted solution was spread on the LB medium plate, and each gradient was spread in three parallels, and cultured at a constant temperature of 37°C for 48 hours, and then the bacillus strains were purified by plate streaking method, and were numbered and stored separately. 122 strains of Bacillus were obtained.

[0040] 2. Strain identification

[0041] According to "Berger Bacteria Identification Handbook" (eighth edition) and "Common Bacteria System Identification Handbook" (edited by Dong Xiuzhu, Cai Miaoying, etc., Beijing: Science Press, 2001.2), the bacterial strain JAASHD was carried out Morphological characteristics, culture characteristics and physiological and biochemical characteristics identification, the specif...

Embodiment 2

[0055] Example 2 Screening of Rice Sheath Blight Efficient Antagonistic Bacillus

[0056] ①Preliminary screening: Prepare PDA plates by using the plate confrontation culture method, use a puncher to punch bacteria cakes with a diameter of 5mm at the edge of Bacillus and rice sheath blight, and transplant them in the center of the opposite sides of the plate respectively, at a constant temperature of 25°C Cultivate and observe the inhibitory effect of Bacillus on pathogenic bacteria day by day.

[0057] ②Re-screening: Re-screen the screened Bacillus strains with high-efficiency antagonistic activity, mainly through temperature resistance, acid and alkali resistance, and drug resistance tests, and screen out Bacillus strains with better tolerance for potted plant control test.

Embodiment 3

[0058] Embodiment 3 Bacillus siamese inhibits other pathogenic bacteria

[0059] The screened Bacillus siamese has inhibitory effects on various plant pathogen indicator bacteria by using the plate confrontation method (Table 3).

[0060] Table 3 Inhibitory effect of Bacillus siamese JAASHD on other pathogenic bacteria

[0061]

[0062]

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Abstract

The invention relates to the field of biological prevention and treatment of plant diseases, in particular to a bacillus siamensis strain and application thereof. The strain is preserved in CGMCC (China General Microbiological Culture Collection Center); the preservation code is CGMCC NO.12903; the preservation time is August 24, 2016. The strain is used for preventing and treating rhizoctonia solani kuhn; the potential adverse influence of chemical drug used in the current stage on the human environment and the rice safety can be avoided. The strain has the characteristics that the growth speed is high; the sporulation quantity is large; the action spectrum is wide; the stress resistance is great; the plant growth is promoted, and the like. Therefore the strain belongs to a biological prevention and treatment preparation with great application prospects.

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a Bacillus siamese strain and application thereof. Background technique [0002] As one of the most important food crops in the world, rice is widely planted, especially in my country. Major diseases of rice include rice sheath blight, bacterial blight and rice blast. As an important disease of rice all over the world, rice sheath blight poses a huge threat to rice safety production and income increase. At present, its occurrence area, occurrence frequency, and yield loss are the first among the three major rice diseases in some rice areas in my country. In particular, the lack of varieties with high resistance to sheath blight in the current rice germplasm resources, the increase in the use of nitrogen fertilizers, the increasing planting density, and the popularization and planting of dwarf rice varieties and hybrid rice varieties have led to the occurrence...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 王景会李达王颖王义生徐文静郑建波牛红红迟燕平苗欣宇李倬林
Owner JILIN ACAD OF AGRI SCI
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