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Liquid phase mobility separating device, control method, and interface used for liquid chromatography-mass spectrometry

A separation device and mobility technology, applied in the field of mass spectrometry, can solve the problems of low ionization efficiency and low abundance

Pending Publication Date: 2018-05-08
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the electrospray method is used to detect mixed samples, there will be ionization competition between the components to be tested, and between the components to be tested and impurities, making it difficult for components with low abundance and low ionization efficiency to be detected

Method used

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  • Liquid phase mobility separating device, control method, and interface used for liquid chromatography-mass spectrometry
  • Liquid phase mobility separating device, control method, and interface used for liquid chromatography-mass spectrometry
  • Liquid phase mobility separating device, control method, and interface used for liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] In this embodiment, the influence of pressure on the sample migration time is analyzed through simulation theoretical calculation and experimental test respectively.

[0090]The simulation parameters are set as follows: the length of the capillary tube is 60cm, the inner diameter of the tube is 75μm, the reverse separation voltage is -10kV or no separation voltage is applied, the viscosity coefficient is 0.89mPa·S, the relative molecular weight of the sample is 1048, with 2 positive charges, the sample, etc. The effective radius is 1.2nm. The separation pressures at both ends of the separation channel are 10mbar, 15mbar, 20mbar, 30mbar, 40mbar, 50mbar respectively. Simulation results such as Figure 6-1 shown. It can be seen that when the reverse separation voltage is applied, as the pressure at both ends of the separation channel increases, the migration time of the substance shortens, and the migration time shortens abruptly from 10mbar to 15mbar, and gradually shor...

Embodiment 2

[0094] In this embodiment, the influence of the separation voltage on the sample migration time is analyzed through simulation theoretical calculation and experimental testing.

[0095] The simulation parameters are set as follows: the length of the capillary tube is 60 cm, the inner diameter of the tube is 75 μm, the separation pressure of the pumped buffer is 30 mbar, the viscosity coefficient is 0.89 mPa S, the relative molecular weight of the sample is 1048, with 2 positive charges, and the equivalent radius of the sample is 1.2nm. The reverse separation voltages are 0V, -100V, -200V, -500V, -1kV, -2kV, -5kV, -10kV, -15kV, -20kV, -25kV, -30kV. The result is as Figure 7-1 As shown, it can be seen that as the separation voltage increases, the migration time of the species gradually prolongs.

[0096] Experimental conditions: use the experimental conditions of the above-mentioned embodiment 1, specifically: the sample is angiotensin II of 1 mg / mL, the buffer solution is 20...

Embodiment 3

[0098] In this embodiment, the effect of the length of the separation capillary on the migration time of the sample is analyzed through simulation theoretical calculation and experimental testing.

[0099] The simulation parameters are set as follows: the inner diameter of the tube is 75 μm, the reverse separation field strength is -300 V / cm, the separation pressure of the pumped buffer solution is 30 mbar, the viscosity coefficient is 0.89 mPa·S, the relative molecular weight of the sample is 1048, with 2 positive charges , the sample equivalent radius is 1.2nm. The capillary lengths are 20cm, 40cm, 60cm, 80cm, and 100cm, respectively. Simulation results such as Figure 8-1 shown. It can be seen that when the separation field strength is constant, as the capillary length increases, the material migration time increases slowly at first, and then increases rapidly.

[0100] Experimental conditions: the sample is a mixed solution of angiotensin II with a final concentration o...

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Abstract

The invention provides a liquid phase mobility separating device, a control method, and an interface used for liquid chromatography-mass spectrometry. The liquid phase mobility separating device comprises a separation capillary, a syringe pump, a sample introduction needle, a separation electrode, and a grounding electrode; one end of the separation capillary is provided with an electrospray point, and the other end is provided with a buffer injection point; the syringe pump is connected with the buffer injection point; the sample introduction needle is connected with the separation capillaryat a position close to the syringe pump; the separation electrode is connected with the syringe pump, or is connected with the separation capillary at a position close to the syringe pump; the grounding electrode is connected with the separation capillary at a position close to the electrospray point. The liquid phase mobility separating device is capable of realizing component separation of a complex sample system, and improving separation effect.

Description

technical field [0001] The invention relates to the field of mass spectrometry, in particular to a liquid-phase mobility separation device, a control method and an interface used with liquid chromatography and mass spectrometry. Background technique [0002] Mass spectrometry is an analytical method that separates and detects samples according to different mass-to-charge ratios (m / z) to identify components and structures. Due to its high specificity and sensitivity, mass spectrometry plays an increasingly important role in the field of biological analysis. [0003] The basic principle of mass spectrometry is to ionize the components in the sample in the ion source, generate ions with different mass-to-charge ratios, and enter the mass analyzer in the form of ion beams. For the detection of liquid samples, the most commonly used ion source is the electrospray ion source. When the electrospray method is used to detect mixed samples, there will be ionization competition betwe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72H01J49/26G01N30/02
CPCG01N30/02G01N30/72H01J49/26G01N27/623G01N27/44765G01N27/44773G01N27/44769G01N30/7266G01N30/463G01N27/4473H01J49/167H01J49/0404G01N33/68
Inventor 徐伟贺木易罗盼
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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