Multifunctional nucleic acid testing device

Abstract

The invention discloses a multifunctional nucleic acid testing device, comprising a base, a transmission mechanism, a liquid drawing mechanism, a suction mechanism and a housing sealed to the base. The base is provided with a plurality of liquid channels, allowing multiple functions to be integrated, such as extraction, amplification and testing of nucleic acid, or amplification and detection forat least one target template extracted. The base and the housing are hermetically fitted to form an independent cavity; the transmission mechanism, the liquid drawing mechanism and the suction mechanism carried by an operating device allow the liquid drawing mechanism to change among the different liquid channels, and sucking a liquid or discharging a liquid from the different liquid channels canbe achieved. The multifunctional nucleic acid testing device is simple in structure, may gain small size, can provide multiple functions integrated for nucleic acid testing, is simple to operate, allows nucleic acid testing to be applied to more medical environments locking professions and experimental conditions, and helps apply and popularize nucleic acid testing.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a multifunctional nucleic acid detection device. Background technique [0002] Nucleic acid amplification detection has been widely used in biomedicine and other fields. In the traditional process of nucleic acid amplification detection, it is common for false positive detection results to be caused by cross-contamination of amplified products. Moreover, most of the templates used in traditional nucleic acid amplification detection methods need to go through an independent sample extraction step, and this step is also likely to cause template cross-contamination and affect the judgment of sample test results. At present, there are some integrated nucleic acid detection devices on the market, such as GeneXpertSystem, etc., which can realize the extraction, amplification and detection of the nucleic acid to be tested in a closed system, which greatly avoids the contamina...

AI Technical Summary

Problems solved by technology

In the traditional process of nucleic acid amplification detection, it is common to have false positive test results due to cross-contamination of amplification products

Moreover, most of the templates used in traditional nucleic acid amplification detection methods need to go through an independent sample extraction step, and this step is also likely to cause template cross-contamination and affect the judgment of sample test results.

At present, there are some integrated nucleic acid detection devices on the market, such as GeneXpertSystem, etc., which can realize the extraction, amplification and detection of the nucleic acid to be tested in a closed system, which greatly avoids the contamination of the sample during the extraction and amplification process. Effectively improve the accuracy and operability of nucleic acid detection, but these detection devices generally have the problems of large size, complex structure, need to use fluorescent probes and high detection cost, and these detection devices are all developed and produced abroad. The equipment is expensive; there are also some miniaturized nucleic acid detection devices, but there is a problem of low detection accuracy during the detection process

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