A modification method for improving immunological competence of milkvetch root-glossy privet fruit polysaccharides
A technology of immune activity and modification method, which is applied in the field of sulfation modification to improve the immune activity of Zhenqi polysaccharide, which can solve the problem of low biological activity of Zhenqi polysaccharide, achieve the effect of weak immune effect and improve immune activity
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[0017] The invention provides a modification method for improving the immune activity of chastostilbene polysaccharide. The steps include: extracting and purifying chastostilbene polysaccharide, suspending the chastostilbene polysaccharide in DMF, adding it to an esterification reagent, and shaking and reacting at 60-95°C 1 to 3 hours, after the reaction is completed, add ice water to terminate the reaction, cool down, and post-process to obtain sulfated chastostilbene polysaccharide; the preparation step of the esterification reagent includes: adding chlorosulfonic acid dropwise to pyridine, stirring and reacting to obtain the esterification reagent, The mass ratio of the chlorosulfonic acid to pyridine is 1:4˜1:8.
[0018] The present invention carried out sulfation modification on two mixed polysaccharides (Zhenqi polysaccharide) for the first time, and established a sulfation modification method, optimized the modification conditions based on the enhancement of immune activ...
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