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Prostate cancer markers and application thereof

A technology of prostate cancer and markers, applied in the field of biomedicine

Active Publication Date: 2018-04-13
徐州维康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the combination of ID1 and STAT3 as molecular markers in prostate cancer

Method used

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  • Prostate cancer markers and application thereof
  • Prostate cancer markers and application thereof
  • Prostate cancer markers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Comparing the relationship between the expression of STAT3 gene and ID1 gene in prostate cancer patients and survival rate

[0039] The Cancer Genome Atlas (TCGA) database contains multiple levels of data information for various tumor types, including: miRNA, mRNA, protein profile, mutation profile, clinical diagnosis information, etc. These data provide a rich resource for tumor data analysis. Therefore, we first analyzed the correlation between STAT3 and ID1 genes and cancer with the help of TCGA database.

[0040] (1) 70 prostate cancer patients were randomly selected from the database, and the prostate tissue of the patients had been detected by RNA-seq to detect the expression level of the whole gene.

[0041] (2) According to the detected expression level of ID1 gene, the patients were divided into two groups with low expression of ID1 and high expression of ID1, 35 people in each group, and the five-year survival rate was compared. and verify whether...

Embodiment 2

[0045] Example 2: Using immunohistochemical staining to compare the differences in the expression of STAT3 and ID1 genes between tumor tissue and normal tissue Materials and methods:

[0046] (1) 12 tumor tissues of prostate cancer patients (including 4 in stage II and 8 in stage IV) were randomly selected, and the tissues of prostate cancer patients were taken to be paraffin-embedded and sectioned for HE staining.

[0047] (2) Prostate cancer patients were randomly selected, and the tissues of the prostate cancer patients were taken for paraffin-embedded sectioning, and immunohistochemical staining for STAT3 and ID1 were performed respectively.

[0048] (3) Randomly select patients with stage II prostate cancer and stage IV prostate cancer, take their tissues for paraffin-embedded sections (including tumor sites and adjacent tissues), and use STAT3 phosphorylation antibody (purchased from Cell Signaling Technology Company, product number 9145) ) and ID1 antibody (purchased fr...

Embodiment 3

[0050] Example 3: Using gene knockout and protein activity inhibitors to compare the effects of different STAT3 and ID1 expression levels on cell proliferation Materials and methods:

[0051] (1) Two different shRNAs (purchased from the Open Biosystems shRNA library of GE Dharmacon, shRNA1 product number V3LMM_519695, shRNA2 product number V3LMM_519694) were used to knock down the ID1 gene, and the degree of down-regulation of ID1 expression in the two cases was compared, and knockdown was selected. The shRNA with better effect was used for subsequent experiments.

[0052] (2), the prostate epithelial cell line TRAMP-C3 (ATCC number CRL-2732 TM , mouse origin, epithelioid adherent growth) cells were divided into four groups, group I was the control group without gene knockdown and inhibitors; group II added STAT3 protein activity inhibitor (SH-4-54, purchased from MCE company, Cat. No. HY-16975); Group III is the ID1 gene knockdown group; Group IV undergoes STAT3 expression i...

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Abstract

The invention relates to prostate cancer markers STAT3 and ID1 and application of a kit which is used for detecting STAT3 and ID1 protein expression levels in prostate cancer detection and diagnosis or prognostic evaluation of patients. As STAT3 and ID1 gene sequences in prostate cancer cells have remarkably high expression, whether the expression of the two genes is high or not has remarkable correlation with clinical pathology parameters; thus, the STAT3 and the ID1 can serve as specific tumor markers or treating targets in early detection of prostate cancer and have important clinical application values.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a group of tissue tumor markers related to prostate cancer and applications thereof. Background technique [0002] Prostate cancer is an important disease that endangers the health of men in my country. In 2012, the incidence rate of prostate cancer in my country's tumor registration areas was 9.92 / 100,000, ranking sixth among male malignant tumors and third among male genitourinary system malignant tumors. The age of onset is at a low level before the age of 55, and gradually increases after the age of 55. The incidence rate increases with age, and the peak age is 70-80 years old. Patients with familial hereditary prostate cancer had a slightly earlier age of onset, and 43% of patients were ≤55 years old. At present, my country has entered an aging society. With the prolongation of population life, the change of diet structure, and the continuous improvement of tumor screeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00
CPCA61K45/00C12Q1/6886C12Q2600/158G01N33/57434G01N33/57484
Inventor 赵蔚何秋萍
Owner 徐州维康生物科技有限公司
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