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Primer set for detecting plasma miR-140-3p of child patient with biliary atresia and detection kit containing same

A detection kit and biliary atresia technology, which are applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of good specificity, high cost, unfavorable promotion and application, etc. The effect of high sensitivity and low price cost

Inactive Publication Date: 2018-04-13
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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Problems solved by technology

Fluorescent quantitative PCR is divided into two parts: dye method and probe method. Although the probe method has good specificity, it needs to design corresponding TaqMan probes for each miRNA, which is costly and not conducive to popularization and application.

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  • Primer set for detecting plasma miR-140-3p of child patient with biliary atresia and detection kit containing same
  • Primer set for detecting plasma miR-140-3p of child patient with biliary atresia and detection kit containing same
  • Primer set for detecting plasma miR-140-3p of child patient with biliary atresia and detection kit containing same

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Embodiment Construction

[0028] The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings. This implementation case is carried out on the premise of the technology of the present invention, and the detailed implementation mode and specific operation process are given now, but the protection scope of the present invention is not limited to the following examples.

[0029] 1. Implement high-throughput sequencing of samples

[0030] 1. Grouping of sequencing samples The "mixed pool" method was used to group and mix samples. The collected 9 cases of BA patients were divided into three groups (A1-3) according to age and gender (3 persons / group), and 9 normal control children were divided into three groups according to age and sex. After age and sex were matched with the case group, they were also divided into three groups, N1~3; the equal volumes (200ul:200ul:200ul) of plasma samples from 3 cases in the group were mixed into 1 sample,...

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Abstract

The invention discloses a primer set for detecting plasma miR-140-3p of a child patient with biliary atresia. The primer set comprises a reverse transcription primer shown in SEQ ID NO:1, a forward upstream primer as shown in SEQ ID NO:2, and a reverse primer as shown in SEQ ID NO:2. The invention further provides a detection kit containing the primer set. The primer set and the kit of fluorescentquantitative PCR for detecting the miR-140-3p, provided by the invention, have high specificity and sensitivity, and can be applied to rapid and specific detection of the miR-140-3p content in plasmain the fluorescent quantitative PCR.

Description

technical field [0001] The invention relates to primers and a kit, in particular to a primer set for detecting plasma miR-140-3p in children with biliary atresia and a detection kit comprising the primer set. Background technique [0002] Biliary atresia (BA) is a common disease that causes cholestatic jaundice in infants and young children. BA is a progressive inflammatory lesion involving the intrahepatic and extrahepatic biliary system, eventually leading to the disappearance of extrahepatic or even extrahepatic bile ducts, liver fibrosis, portal hypertension and other complications, which ultimately endanger the lives of children. If early and timely surgical treatment is not given, the natural mortality rate can reach 100%. Therefore, early screening, diagnosis and assessment of liver fibrosis are of great significance for the prognosis of children with BA and the effective reduction of neonatal mortality in the region. However, there is still a lack of specific and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6883C12Q2600/178C12Q2563/107C12Q2545/114
Inventor 刘海英彭小芳庞舒尹陈翊陈奕豪陈峥嵘
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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