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A kit for early diagnosis of neoplastic disease, a method and applications of the kit

A technology of early diagnosis and kit, which is applied in the field of diagnostic reagents for tumor diseases, can solve the problems of time-consuming and labor-intensive, specific capture and detection of tumor cancer exosomes, and achieve good application prospects

Inactive Publication Date: 2018-04-10
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the specific capture and detection of cancer exosomes is still difficult and time-consuming

Method used

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  • A kit for early diagnosis of neoplastic disease, a method and applications of the kit
  • A kit for early diagnosis of neoplastic disease, a method and applications of the kit
  • A kit for early diagnosis of neoplastic disease, a method and applications of the kit

Examples

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Embodiment 1

[0076] Taking liver cancer as an example, a method for preparing a kit for early diagnosis of liver cancer tumor disease and a detection method using the kit are provided below. The preparation method of the kit includes the following steps:

[0077] S1. Preparation of a specific capture system for tumor exosomes, including a magnetic gold nanosphere complex solution with a ferroferric oxide core modified by tumor exosomes specific ligands and a gold nanocage modified by exosomes specific ligands Solution; In this example 1, tumor exosomes refer to GPC3 high expression liver cancer exosomes GPC 3+ crExos;

[0078] S11, preparation of magnetic gold nanospheres

[0079] S111, to the 0.2nM NaCl solution at 65℃ and pH 8.5, add 12mM (NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O solution 6mL, quickly stir the solution, and then quickly add freshly prepared 4.17mM H 2 O 2 Solution and make H in solution 2 O 2 With Fe 2+ The molar ratio is 1:8, and the (NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O, regulate Fe 3 O 4 The pa...

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Abstract

The invention belongs to the technical field of neoplastic disease diagnosis, and discloses a kit for early diagnosis of neoplastic disease, a method and applications of the kit. The kit includes a specific capturing and detecting system for a tumor exosome and a molecular beacon fluorescent detection system. The specific capturing and detecting system for the tumor exosome includes a solution ofa magnetic gold nanosphere composite modified by a tumor exosome specific ligand and adopting ferric ferrous oxide as a core, and a gold nanocage solution modified by the exosome specific ligand. Themolecular beacon fluorescent detection system includes a molecular beacon probe provided with a fluorescent group, fluorescent amplification substrate hybrid chains provided with fluorescent groups and a buffer liquid. The kit and the method combine tumor exosome specific capturing and analysis and a molecular beacon fluorescent detection method, and greatly increases accuracy and reliability of early diagnosis of tumor.

Description

Technical field [0001] The invention belongs to the technical field of diagnostic reagents for tumor diseases, and specifically relates to a kit for early diagnosis of tumor diseases, preparation and detection methods and applications. Background technique [0002] At present, the diagnosis of many diseases still faces severe challenges. Tumor is one of the main causes of death, and it is also a hot and difficult point of current research. Generally, the symptoms of cancer are not obvious in the early stage of cancer, and it is difficult to detect and detect. When the cancer is diagnosed, the condition has often progressed to the middle and advanced stages, and the prognosis is poor and the survival period is short. Therefore, early detection and timely treatment are the key to effective prevention and treatment of tumor diseases. [0003] Exosomes are nano-sized vesicles with a diameter of about 50-150 nm, which exist in blood and other body fluids. In 2015, “Nature” reported ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/682
CPCC12Q1/682C12Q2563/137C12Q2563/107C12Q2525/301C12Q2525/207
Inventor 胡玉荣吉梦飞李慧丽袁金秀段少峰徐鑫黄胜楠
Owner ZHENGZHOU UNIV
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