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Rate type fluorescent probe used for mercuric ion detection and preparing method thereof

A fluorescent probe and detection method technology, applied in the field of mercury ion detection, can solve the problems of cumbersome material steps, complex reactions, pollution of the environment, etc., and achieve the effects of high sensitivity, low detection limit, and low energy consumption

Inactive Publication Date: 2018-04-06
SICHUAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The steps of materials synthesized by this method are very cumbersome, many organic reagents are used, the reaction is complicated, and most of the organic reagents are harmful to the human body and pollute the environment

Method used

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  • Rate type fluorescent probe used for mercuric ion detection and preparing method thereof
  • Rate type fluorescent probe used for mercuric ion detection and preparing method thereof
  • Rate type fluorescent probe used for mercuric ion detection and preparing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A ratiometric fluorescent probe for mercury ion detection, the probe is composed of gold / silver nano-clusters of dual-emission nanomaterials wrapped by bovine serum albumin, and its fluorescence emission wavelengths are 400-550nm and 580-680nm.

Embodiment 2

[0032] A ratiometric fluorescent probe for mercury ion detection, the probe is composed of gold / silver nano-clusters of dual-emission nanomaterials wrapped by bovine serum albumin, and its fluorescence emission wavelengths are 400-550nm and 580-680nm.

[0033] The gold / silver molar ratio in the fluorescent probe is 1:1.

[0034] The mass ratio of bovine serum albumin to silver ions in the fluorescent probe is 40:1.

Embodiment 3

[0036] A ratiometric fluorescent probe for mercury ion detection, the probe is composed of gold / silver nano-clusters of dual-emission nanomaterials wrapped by bovine serum albumin, and its fluorescence emission wavelengths are 400-550nm and 580-680nm.

[0037] The gold / silver molar ratio in the fluorescent probe is 1:1.

[0038] The mass ratio of bovine serum albumin to silver ions in the fluorescent probe is 50:1.

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Abstract

The invention provides a rate type fluorescent probe used for mercuric ion detection. The probe is formed by dual-emitter nanometer material gold / silver nanoclusters wrapped by bovine serum albumin, and the fluorescence-emission wavelength is 400-550 nm and 580-680 nm. The dual-emitter nanometer material gold / silver nanoclusters are synthesized through a one-pot method by using the bovine serum albumin (BSA) as a reducing agent and a stabilizer. The synthesized gold / silver nanoclusters (Au / Ag NCs) have two emission peaks, wherein Ag NCs serves as a reference signal Au NCs serves as a responsesignal and is used for selective recognition of Hg2+. When the dual-emitter gold / silver nanoclusters serve as the rate type fluorescent probe, the Ag NCs fluorescent signal intensity is kept unchangedbasically, and Au NCs is selectively combined with Hg2+ to cause fluorescence quenching. The method is quick, simple, low in detection limit, wide in linear range and high in selectivity.

Description

technical field [0001] The invention belongs to the technical field of mercury ion detection, in particular to a ratio fluorescent probe for mercury ion detection. Background technique [0002] With the rapid development of the economy, the pollution of heavy metals in the environment is becoming more and more serious, seriously endangering human health and environmental safety. Mercury ions (Hg 2+ ) as a heavy metal ion not only pollutes the environment but also has major hazards to the human body. It can cause growth retardation and some health problems, such as damage to the brain and nervous system, causing cognitive bias, Minamata disease, causing movement disorders and affecting the endocrine system. At the same time, mercury ions (Hg 2+ ) The pollutants represented by ) will not decompose quickly once they enter the environment, but remain in the soil, water, and air for a long time, resulting in enduring pollution. Therefore, it is of great significance to find an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 熊小莉黄科代蕊邓文清
Owner SICHUAN NORMAL UNIVERSITY
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