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Cremastra appendiculata seed tissue culture and propagation method

A seed group, rhododendron orchid technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of scarce and expensive materials, inconvenient storage and transportation, etc., and achieve the effect of good plant growth, convenient transportation, and well-developed root system

Active Publication Date: 2018-04-06
MIANYANG SHUCHUANG AGRI TECH CO LTD
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Problems solved by technology

[0003] In summary, the existing problems in the prior art are: the rapid propagation technology of pseudobulbs has rare and expensive materials, and inconvenient storage and transportation.

Method used

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  • Cremastra appendiculata seed tissue culture and propagation method

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Embodiment Construction

[0019] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0020] The invention solves the technical problem of using seeds for propagation by developing a tissue culture method and a medium formula of rhododendron orchid seeds.

[0021] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0022] Such as figure 1 Shown, the rhododendron orchid seed tissue culture rapid propagation method that the embodiment of the present invention provides comprises the following steps:

[0023] S101: Collect the mature seeds of Rhododendron orchid;

[0024] S102: Rinse under running water for 10 minutes, peel off ...

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Abstract

The invention belongs to the technical field of medicinal herb propagation and discloses a cremastra appendiculata seed tissue culture and propagation method. The method comprises the following steps:flushing with running water for 10 minutes, and stripping seeds; disinfecting by using 0.1% of mercury bichloride 10min+70% of alcohol 10s; inoculating into MS+2, 4-D1mg / L+BA 1mg / L callus induction;transferring into 1 / 2MS+NAA 1mg / L+BA 1mg / L callus to realize seedling differentiation; culturing under the conditions of 24+ / -1 DEG C and 2000-2100lx, and illuminating 8-10 hours per day; and placingculture bottles of tissue culture seedlings under room-temperature natural lighting conditions for hardening seedlings, cleaning with clean water, planting into a pre-disinfected substrate, and splitcharging by a feeding block. The induction rate can reach 55% within 50 days, the seedlings are grown within 40 days after culture, the survival rate of the seedlings is 83%, the new roots grow earlier, the root system is developed and strong, and plant growth is good.

Description

technical field [0001] The invention belongs to the technical field of medicinal herbal plant propagation, and in particular relates to a method for tissue culture and rapid propagation of rhododendron orchid seeds. Background technique [0002] Rhododendron orchid (Cremastra appendiculata), also known as abacus seven, three hoops, Chaotian Yizhuxiang, etc., is a perennial medicinal herb of the genus Rhododendron orchid in the family Orchidaceae. Important Chinese herbal medicines are in short supply. Due to people's long-term plundering and digging, its wild resources are becoming increasingly depleted. The embryo of rhododendron orchid seeds is not fully developed, and it is not easy to germinate. It is difficult to reproduce pseudobulbs under natural conditions. Only one new pseudobulb can be produced in one year of growth, so the propagation speed is extremely slow, making it difficult to carry out artificial planting on a large scale. Tissue culture rapid propagation ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡进耀胡麓汐向莉王虹别鹏飞
Owner MIANYANG SHUCHUANG AGRI TECH CO LTD
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