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Method for tissue culture and rapid propagation of Cymbidium tigrinum

A technology for tissue culture, rapid propagation and bark tongue, which is applied in the field of plant tissue culture, can solve the problems of chemical disinfectant sensitivity, easy infection of bark tongue, and tender stem tips, etc., and achieves the effect of increasing disinfection.

Inactive Publication Date: 2018-04-06
秦素梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, some scholars have studied the method of using zebra quill tips as explants for tissue culture to obtain zebra quilla seedlings, but the zebra quill tips are easy to be infected with bacteria, and the stem tips are tender and sensitive to chemical disinfectants. , vulnerable to damage, resulting in low proliferation and differentiation rates during the culture process; in addition, due to the lack of related research, no medium that is more suitable for the growth of shoot tip differentiated tissues has been found, resulting in long culture periods and poor culture effects during tissue culture. unsatisfactory

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  • Method for tissue culture and rapid propagation of Cymbidium tigrinum
  • Method for tissue culture and rapid propagation of Cymbidium tigrinum

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Embodiment 1

[0030] A method for tissue culture rapid propagation of zebra quilla, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0031](1) Disinfection of explants: collection length is 5-7cm zebra pseudobulbs as explants, at first rinse with running water, then peel off the surface part of the leaves after soaking in 75% alcohol for 1min with a volume concentration, then After soaking in the plant disinfectant for 8 minutes, continue to peel off part of the leaves, then continue to soak in the plant disinfectant for 5 minutes, rinse twice with sterile water, and finally move into the ultra-clean workbench, and peel off the remaining leaves under the dissecting microscope. The leaves of the leaves are exposed to the growth point, and the shoot apical meristem with a size of 0.5-2mm is cut with a scalpel; the plant disinfectant is made by mixing the following raw materials in parts by weight: 0.05 parts of acetylshik...

Embodiment 2

[0037] A method for tissue culture rapid propagation of zebra quilla, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0038] (1) Disinfection of explants: collection length is the 5-7cm pseudobulb of zebra quill as explants, at first rinses with running water, then peels off surface part blades after 75% alcohol soaking 1.5min with volume concentration, Then soak in the plant disinfectant for 12 minutes and continue to peel off part of the leaves, then continue to soak in the plant disinfectant for 8 minutes, rinse with sterile water for 3 times, finally move into the ultra-clean workbench, and peel off the remaining leaves under the dissecting mirror. The lower leaves expose the growth point, and cut the shoot tip meristem with a size of 0.5-2mm with a scalpel; the plant disinfectant is made by mixing the following raw materials by weight: 0.06 parts of acetylshikonin, 9 parts of cactus juice, Gardenia ...

Embodiment 3

[0044] A method for tissue culture rapid propagation of zebra quilla, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0045] (1) Disinfection of explant: collection length is the pseudobulb of zebra quilla of 5-7cm as explant, at first washes with running water, then peels off surface part blade after soaking 2min with the alcohol of 75% volume concentration, then After soaking in the plant disinfectant for 15 minutes, continue to peel off some leaves, then continue to soak in the plant disinfectant for 10 minutes, rinse 4 times with sterile water, and finally move into the ultra-clean workbench, and peel off the remaining leaves under the dissecting microscope. The leaves of the plant are exposed to the growth point, and the shoot tip meristem of 0.5-2mm size is cut with a scalpel; the plant disinfectant is made by mixing the following raw materials in parts by weight: 0.08 parts of acetylshikonin, 10 pa...

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Abstract

The invention provides a method for tissue culture and rapid propagation of Cymbidium tigrinum, belonging to the technical field of plant tissue culture. The method comprises the following steps: explant disinfection, induction culture, subculture, strong seedling culture, etc., wherein the step of explant disinfection adopts a disinfection manner of combining ethyl alcohol with a plant disinfectant; the plant disinfectant comprises effective components of acetylshikonin, a cactus juice and a Gardenia jasminoides Ellis extract; and a Moringa oleifera leaf juice and an okra juice used as nutrients are added into the culture medium used in various culture phases. The method provided by the invention has little harm to an explant; meanwhile, by adoption of a formula of the culture medium provided by the invention, the induced differentiation rate of the Cymbidium tigrinum and the propagation coefficient and the rooting rate of the explant can be significantly improved; the period of culture is shortened; and the quality of a tissue culture seedling of the Cymbidium tigrinum is improved.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of zebra quillia. 【Background technique】 [0002] Spotted tongue is a kind of orchid, named for its unique flower shape, and it is a famous product in orchids. Sepals and petals are yellowish green, slightly reddish-brown halo, with purple-brown spots at the base, labial petals are white, and turn pink after pollination; petals are slightly shorter than sepals; labial petals are nearly obovate, 3-lobed. As a national key protected plant, it is the most rare orchid variety in family breeding. But because of its noble and pure cultural character, it is favored by literati and refined scholars. [0003] For a long time, zebra tongues have been mainly propagated by ramets, but the propagation speed is slow. If you want to get more plants in a short period of time, you can use tissue culture to propagate. There are fe...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01N65/08A01N37/02A01P1/00
CPCA01H4/008A01H4/00A01H4/001A01N37/02A01N65/00A01N65/08
Inventor 秦素梅
Owner 秦素梅
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