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Fluorescence Background Subtraction Method Based on Raman Spectroscopy

A technology of Raman spectroscopy and fluorescence background, which is applied in the field of fluorescence background subtraction of Raman spectroscopy, can solve the problems of high cost and narrow application range, and achieve the effects of low price, wide application range and wide application range.

Active Publication Date: 2020-02-18
西安电子科技大学青岛计算技术研究院
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Problems solved by technology

[0006] The purpose of the present invention is to provide a Raman spectrum fluorescence background subtraction method based on spectrum removal, which can solve the problems of high cost and narrow application range in existing methods

Method used

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  • Fluorescence Background Subtraction Method Based on Raman Spectroscopy
  • Fluorescence Background Subtraction Method Based on Raman Spectroscopy
  • Fluorescence Background Subtraction Method Based on Raman Spectroscopy

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Embodiment Construction

[0031] The following will clearly and completely describe the technical solutions of the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0032] figure 1 Be the flowchart of the method of the present invention, as figure 1 Shown, the inventive method comprises the following steps:

[0033] Collect the original Raman spectrum I(λ) and Raman shift λ of the sample, the Raman shift is the abscissa in the Raman spectrum, and use the Raman spectroscopy device to collect the original Raman spectrum I(λ) and Raman shift λ of the sample , the original Raman spectrum I(λ) generally has a strong fluoresc...

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Abstract

An absorbance division-based Raman spectroscopy fluorescence background subtraction method comprises the following steps: collecting the original Raman spectrum and the Raman shift of a sample; acquiring a shift Raman spectrum, a matching Raman spectrum and a matching Raman shift; calculating a shift Raman absorbance division based on the obtained matching Raman spectrum and shift Raman spectrum;observing the positions of positive and negative peaks in the shift Raman absorbance division to determine a Raman spectrum peak region, and dividing a non-Raman spectrum peak region in the matching Raman spectrum into several spectrum regions; removing the Raman spectrum peak region from the matching Raman spectrum, and performing piecewise fitting on the Raman spectrum peak region removed matching Raman spectrum according to the division result of the spectrum regions in order to obtain an estimated fluorescence background; and calculating the fluorescence background subtracted Raman spectrum according to the matching Raman spectrum and the estimated fluorescence background. The method using the shift absorbance division to realize the subtraction of the Raman spectrum has the characteristics of wide application range, simple algorithm, low price and small personnel error.

Description

technical field [0001] The invention belongs to the technical field of data processing, in particular to a fluorescence background subtraction method of Raman spectroscopy. Background technique [0002] In the practical application of Raman spectroscopy, Raman spectroscopy is often accompanied by a strong fluorescent background. For example, the Raman spectra of biological / medical related samples such as bone fluid, human plasma, human skin, and pharmaceuticals are accompanied by a strong fluorescent background. The presence of the fluorescent background will cause the Raman signal to be submerged in the fluorescent background. In order to obtain the Raman signal, it is necessary to subtract the fluorescence background of the Raman spectrum. Many methods have been proposed and used to subtract the strong fluorescence background in Raman spectra. Commonly used fluorescence background subtraction methods mainly include the following: methods based on sample pretreatment, met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 林珂王志强鞠思文张睿挺
Owner 西安电子科技大学青岛计算技术研究院
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