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Method for extracting ceramide from chestnut shells

A technology of ceramide and chestnut shell, which is applied in the field of fermentation industry, can solve the problems of difficult extraction and achieve the effects of short fermentation cycle, reduced environmental impact and simple operation

Active Publication Date: 2018-03-23
JINZHAI LIMIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, it has been discovered that the molecular structure of ceramide is contained in the chestnut shell, but how to extract it is still a big problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting ceramide from chestnut shells comprises the following steps:

[0027] (1) Soak the chestnut shells in salt water for 1 minute, then wash them with water. The concentration of the salt water is 1.5% by weight, and the dosage is twice the weight of the chestnut shells, and then crushed until the mesh size is less than 1mm. Add the weight of chestnut shells to the chestnut shell powder 3 times the amount of water, add 1% of the chestnut shell weight Lactobacillus acidophilus to ferment, control the fermentation temperature at 34°C, and ferment for 72 hours;

[0028] (2) Centrifuge the fermented liquid obtained in step (1) at high speed, and extract the supernatant for later use;

[0029] (3) Sterilize the clear liquid obtained in step (2) by filtering through a microporous membrane with a pore size of 0.5 μm;

[0030] (4) Use the HPD100 macroporous resin continuous chromatographic column for the filtrate obtained in step (3), first use ethanol with...

Embodiment 2

[0032] A method for extracting ceramide from chestnut shells comprises the following steps:

[0033] (1) Soak chestnut shells in salt water for 2 minutes, and then wash them with water. The weight percentage concentration of salt water is 1.2%, and the dosage is 3 times the weight of chestnut shells, and then crushed until the mesh size is less than 1mm. Add the weight of chestnut shells to chestnut shell powder 4 times the water, add 2% of chestnut shell weight Streptococcus lactis to ferment, control the fermentation temperature at 30°C, and ferment for 60 hours;

[0034] (2) Centrifuge the fermented liquid obtained in step (1) at high speed, and extract the supernatant for later use;

[0035] (3) Filtering and sterilizing the clear liquid obtained in step (2) through a microporous membrane with a pore size of 1 μm;

[0036] (4) Use the DA201 macroporous resin continuous chromatographic column for the filtrate obtained in step (3), first use ethanol with a volume concentrat...

Embodiment 3

[0038] A method for extracting ceramide from chestnut shells comprises the following steps:

[0039] (1) Soak the chestnut shells in salt water for 4 minutes, then wash them with water. The weight percentage concentration of the salt water is 0.8%, and the dosage is 4 times the weight of the chestnut shells, and then crushed until the mesh size is less than 1mm. Add the weight of chestnut shells to the chestnut shell powder 4 times the amount of water, add 4% of the chestnut shell weight Leuconostoc enterococcus to ferment, control the fermentation temperature at 28°C, and ferment for 36 hours;

[0040] (2) Centrifuge the fermented liquid obtained in step (1) at high speed, and extract the supernatant for later use;

[0041] (3) Filtering and sterilizing the clear liquid obtained in step (2) through a microporous membrane with a pore size of 0.5 μm;

[0042] (4) Use the HPD100 macroporous resin continuous chromatographic column for the filtrate obtained in step (3), first use...

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PUM

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Abstract

The invention discloses a method for extracting ceramide from chestnut shells. The method comprises the following steps: cleaning chestnut shells, crushing the cleaned chestnut shells, adding water with a weight of 3-5 times of the weight of the chestnut shells, adding lactic acid bacteria for fermentation, carrying out centrifugal separation on the obtained fermentation liquor, taking the clear liquor for later use, filtering and sterilizing the obtained clear liquor through a millipore filter membrane, and carrying out continuous chromatographic selection separation on the obtained filtrateto obtain the ceramide. According to the invention, the lactic acid bacteria are used for fermentation of the chestnut shells, and a large amount of ceramide substances can be secreted into the fermentation liquor, so that no derivatization process is needed, the operation is simple, the extraction is convenient, the fermentation period is short, the effect is quick, and the limitation by regionalclimate is avoided. The waste chestnut shells are turned into wealth, and resources are fully utilized. According to the process used for producing the ceramide, the solvent can be repeatedly used, the environmental influence is reduced, and the economic cost is reduced.

Description

technical field [0001] The invention belongs to the field of fermentation industry, and in particular relates to a method for extracting ceramide from chestnut shells. Background technique [0002] Chestnut, known as the "King of Dried Fruits", has another name called chestnut, big chestnut, and chestnut. Chestnut nuts are purple-brown, covered with yellow-brown hairs, or nearly smooth, with light yellow flesh, sweet and delicious taste. Chestnuts are suitable for both raw and fried food. Stir-fried chestnuts with sugar and roasted chicken are fragrant and delicious. They can be ground into powder, and can also be made into various dishes, cakes, canned food, etc. But people often discard chestnut shell when making processing, edible chestnut, cause waste. [0003] Chestnut husk is the exocarp of chestnut. It has sweet, astringent and flat medicinal properties, and has the effects of reducing adverse reactions and stopping bleeding. In recent years, it has been discovered...

Claims

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Application Information

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IPC IPC(8): C07C231/24C07C233/20C07C235/08
CPCC07C231/24C07C233/20C07C235/08
Inventor 赵学群毛建卫张英淘吴善良
Owner JINZHAI LIMIN BIOTECH CO LTD
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