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Application of ethanol extract of bean and taro tubers in reducing lipid deposition in hepatic cells

A technology of ethanol extraction and lipid deposition, which is applied in the field of medicine, can solve the problems of reducing the body's immunity and easy rebound, and achieve the effect of reducing lipid deposition in LO2 cells and having a good development prospect

Active Publication Date: 2021-01-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the other weight loss and fat reduction methods also have the disadvantages of lowering the body's immunity and easy rebound.

Method used

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  • Application of ethanol extract of bean and taro tubers in reducing lipid deposition in hepatic cells
  • Application of ethanol extract of bean and taro tubers in reducing lipid deposition in hepatic cells
  • Application of ethanol extract of bean and taro tubers in reducing lipid deposition in hepatic cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, a kind of preparation method of bean taro tuber ethanol extract, carries out following steps successively:

[0059] 1), in the ratio of 1g:5ml, mix the bean and taro tubers with 95% ethanol containing 0.1mol / L hydrochloric acid, and use a juice extractor to beat; After extracting for 240 ± 10 minutes, the extract was centrifuged (4000 rpm for 30 minutes), and the centrifuged filter residue replaced the potato tubers in the above steps to repeat the beating, ultrasonic extraction and centrifugation of the above steps; the number of repetitions was 3 times;

[0060] The supernatants obtained by the above 3 times of centrifugation were combined to carry out the following step 2).

[0061] 2), the obtained supernatant was concentrated by rotary evaporation in a rotary evaporator (the process parameters set by the rotary evaporation were temperature 38°C, vacuum degree -0.09MPa) until it was 10% of the original volume;

[0062] 3), the concentrated solution ob...

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Abstract

The invention discloses an application of ethanol extract of bean and taro tubers in reducing lipid deposition in liver cells: it is used for preparing medicine and health food for treating or preventing non-alcoholic fatty liver. The preparation method of the ethanol extract of bean taro tubers comprises the following steps: mixing bean taro tubers with 95% ethanol containing 0.1mol / L hydrochloric acid, beating, ultrasonic extraction, and centrifugation to obtain a supernatant; concentrating the supernatant , the obtained concentrated solution is separated and purified by macroporous resin; 1% formic acid aqueous solution with volume concentration is used as eluent, and methanol aqueous solution containing 0.2% formic acid is used as eluent to collect the eluate; the eluate is concentrated to obtain The slurry concentrate is pre-frozen at -70--90°C, and then dried into powder by a vacuum freeze dryer to obtain the ethanol extract of bean and taro tubers.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the preparation of the ethanol extract of bean and taro tubers and its application in the preparation of health food and medicine for the prevention and treatment of non-alcoholic fatty liver; at the same time, it relates to the reduction of oleic acid-induced lipid deposition in LO2 cells in the prevention and treatment of Application in nonalcoholic fatty liver disease. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P1/16A61P3/06A23L33/105
CPCA23V2002/00A61K36/48A61K2236/333A61K2236/55A23L33/105A23V2200/30A23V2250/21
Inventor 郑晓冬楚强刘阳阳余露霜李永璐
Owner ZHEJIANG UNIV
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