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SNP (single nucleotide polymorphism) molecular marker with influence on birth weight characters of pigs and application of SNP molecular marker

A technology of molecular markers and traits, which is applied in the field of molecular biotechnology and molecular markers, can solve the problems of little effect and achieve the effect of increasing birth weight, increasing genetic progress, and improving enterprise production efficiency

Active Publication Date: 2018-03-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Birth weight is a trait that is difficult to trace and track continuously. Traditional breeding methods have little effect on its selection, but marker assisted selection (MAS) breaks this technical barrier

Method used

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  • SNP (single nucleotide polymorphism) molecular marker with influence on birth weight characters of pigs and application of SNP molecular marker
  • SNP (single nucleotide polymorphism) molecular marker with influence on birth weight characters of pigs and application of SNP molecular marker
  • SNP (single nucleotide polymorphism) molecular marker with influence on birth weight characters of pigs and application of SNP molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In the embodiment, a total of 648 purebred Large White pigs were used; wherein, the body weight of each individual was determined at 2 hours after birth as the phenotype value of the birth weight trait. The above experiment was carried out in the Shahu Breeding Pig Farm of Guangdong Wen's Food Group Co., Ltd. All sows adopted uniform feeding and management standards throughout the pregnancy and postpartum lactation period.

[0037] (1) The method of extracting DNA from large white pig ear-like tissue refers to the marked phenol-chloroform method to extract whole-genome DNA. The DNA quality and concentration of the purebred Large White pig population were detected by Nanodrop-ND1000 spectrophotometer. A260 / 280 ratio of 1.8 to 2.0, and A260 / 230 ratio of 1.7 to 1.9 are judged as qualified. Finally, the qualified DNA samples were uniformly diluted to 50 ng / μl.

[0038] (2) Genotype detection of 80K SNP in the whole pig genome: GeneSeek Genomic Profiler Porcine 80KSNP typi...

Embodiment 2

[0044] (1) Amplification of the target fragment containing the SNP site significantly related to the birth weight of Large White pig The target fragment is a 926bp nucleotide sequence in chromosome 8, and the upstream and downstream primers for sequence amplification are:

[0045] Upstream primer PCR-F: 5'-GACTGACCTAAGGGATG-3';

[0046] Downstream primer PCR-R: 5'-CCTGGGAGAATAGCAT-3';

[0047] (2) PCR amplification system and condition setting

[0048] Configure a 20 μL system, including 3.5 μL of DNA sample, 0.6 μL of upstream primer, 0.6 μL of downstream primer, 10 mL of PCR mix, ddH 2 O 5.3 μL, the PCR conditions were pre-denaturation at 98°C for 2 min, denaturation at 98°C for 10 s, annealing at 56.5°C for 15 s, extension at 72°C for 40 s, a total of 35 cycles, and a final extension at 72°C for 10 min.

[0049] (3) Identification by DNA sequence sequencing: sequence sequencing was carried out in Shenzhen Huada Gene Technology Co., Ltd., and the positive and negative reac...

Embodiment 3

[0053] Example 3 Molecular marker SNP site g.138797629 T>C effect analysis

[0054] The present invention provides a SNP marker that can significantly increase the primary weight of large white pigs and their synthetic lines. Using the SNP for marker-assisted selection can greatly increase the primary heavy breeding process of large white pigs and their synthetic lines. All the CC-type individuals of the molecular markers affecting pig birth weight traits of the present invention are bred into TT-type individuals, and the survival rate of piglets can be increased by 2 percentage points. This shows that the excellent birth weight performance has the potential to provide benefits for the pig industry. huge. In this SNP-marked individual, there is a significant difference in birth weight between TT-type individuals and CC-type individuals (P<0.01). By optimizing the dominant allele (T) of this SNP in Large White pigs and their synthetic lines, it can finally achieve The purpose ...

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Abstract

The invention belongs to the field of molecular biological technologies and molecular marker technologies, and particularly relates to an SNP (single nucleotide polymorphism) molecular marker with influence on birth weight characters of pigs and application of the SNP molecular marker. SNP loci of the SNP molecular marker with the influence on the birth weight characters of the pigs correspond tomutation of 138797629th nucleotide loci T C on eighth chromosomes in 10.2 versions of international pig reference genomes and are named g138797629 T C. The invention further provides a primer for identifying the SNP molecular marker. The SNP molecular marker, the application and the primer have the advantages that efficient and accurate molecular marker-assisted breeding technologies can be established by the aid of the SNP molecular marker and the primer, dominant allelomorphic genes of the SNP molecular marker can be optimized, genetic progress of the birth weight characters of the large white pigs can be improved, the survival rate of piglets can be increased, the breeding time for the birth weight characters of the large white pigs can be shortened, accordingly, the breeding pig breeding economic benefits can be effectively increased, the production costs of enterprises can be reduced, and the core competitiveness can be improved.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and in particular relates to a SNP molecular marker affecting pig birth weight traits and its application. Background technique [0002] Over the past few decades, the swine industry has been working to improve pig reproductive performance such as litter size. However, with the continuous improvement of reproductive performance such as litter size, the problems of piglet weight differences and the proportion of low birth weight pigs gradually increasing have become increasingly prominent. Many studies have shown that birth weight has a great influence on the growth traits and carcass traits of pigs. Low birth weight pigs have lower carcass leanness, more fat, less muscle fiber and reach slaughter weight later than high birth weight pigs. However, low birth weight of piglets will lead to increased mortality and culling rates of piglets. At the same time, t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨杰吴珍芳付帝生全建平杨明郑恩琴蔡更元
Owner SOUTH CHINA AGRI UNIV
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