Fast separation method of wild edible fungi

Abstract

The invention discloses a fast separation method of wild edible fungi. The selected raw materials (including pleurotus djamor, agrocybe aegerita, hericium erinaceus, hypsizygus marmoreus and glossy ganoderma) are widely planted in China; the collection is convenient; the plants belong to different species and have relatively high representativeness. The selected raw materials have very high edibleand medicinal values and also have higher activity in the existing research, so that the raw materials have the further development and utilization potential. An efficient wild fungi tissue isolationmethod is built; a conventional tissue isolation method can easily infect bacteria; the time and the labor are wasted; the PCR (polymerase chain reaction) sequencing result is authenticated in a morphology manner; the equal quantity of triadimefon with the concentration being 1mg/L and gentamicin with the concentration being 40mg/L are respectively added on the first day and the second day of fermentation; the edible fungi with high yield and highest purity can be obtained.

Description

technical field [0001] The invention relates to a rapid separation method of wild edible fungi, belonging to the technical field of separation of wild edible fungi. Background technique [0002] Due to its unique landform and environmental characteristics, Southwest my country contains a large number of rare plants, animals and edible fungi. Today, the edible and medicinal value of edible fungi has been widely recognized around the world, especially in China, India, Japan and Korea, where these edible fungi have become important ingredients in traditional Chinese medicine, and at least 270 edible fungi have been studied and confirmed to have biological active. At the same time, edible fungi are low in energy and fat content, but rich in protein, carbohydrates and dietary fiber nutrients. Since fungal cells are rich in non-starch polysaccharides, among which β-glucose is the most functional part, mushrooms can As a potential source of dietary fiber, in addition, edible fung...

AI Technical Summary

Problems solved by technology

Bacteria and mold pollution will directly affect the yield and purity of edible fungi, resulting in serious economic losses and research mistakes. Therefore, it is important to screen out high-efficiency, low-toxicity, and strong selectivity fungicides to effectively prevent and control mold pollution. One of the urgent problems to be solved in production

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