Method for quantitative analysis of sucralose content through combination of mid-infrared spectroscopy and vector angle
A technology of sucralose and infrared spectroscopy, applied in the field of quantitative analysis of compound infrared spectroscopy, to achieve rapid and accurate determination, independent of spectral characteristic response, and low detection limit
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[0045] Experimental Instruments and Reagents
[0046] Frontier Fourier Transform Infrared Spectrometer (Perkin Elmer, USA), Agilent1200 High Performance Liquid Chromatography (Agilent, USA), WS70-1 Far Infrared Fast Drying Oven, CP214 Electronic Analytical Balance.
[0047] Sucralose (sucralose) reference substance (99.99%, by HPLC), sucralose-6-ethyl ester (sucralose-6-ethyl ester) reference substance (96.5%, by HPLC), KBr (AR) , deacylated sample (self-made, containing sucralose, sucralose-6-ethyl ester, a small amount of other by-products such as methyl acetate, considered here as a multi-component sample)
[0048] Serial sample preparation
[0049] Prepare the KBr tablet of the sucralose reference substance and the sucralose-6-ethyl ester reference substance: Accurately weigh the reference substance and add KBr, mix well and then compress the tablet, the numbers are S 0 , S 10 ;
[0050] Prepare the KBr tablet of the two-component sample: the sucralose-6-ethyl ester and ...
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