Mixed polymer solution for capillary electrophoresis and method for preparing mixed polymer solution

A hybrid polymer and capillary electrophoresis technology, which is applied in biochemical equipment and methods, DNA preparation, microbiological determination/inspection, etc., can solve the problems that are difficult to achieve in the laboratory, cumbersome synthesis steps, and large molecular weight requirements, and achieve good results. The effect of DNA separation ability, simple preparation method and great economic benefits

Inactive Publication Date: 2018-02-23
THE FIRST RES INST OF MIN OF PUBLIC SECURITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the synthesis steps of the two inventions are cumbersome, and the molecular weight requirements are relatively large, which is difficult to achieve in the laboratory

Method used

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  • Mixed polymer solution for capillary electrophoresis and method for preparing mixed polymer solution
  • Mixed polymer solution for capillary electrophoresis and method for preparing mixed polymer solution
  • Mixed polymer solution for capillary electrophoresis and method for preparing mixed polymer solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the preparation of polyacrylamide

[0032] Add 222ml of deionized water and 6.55ml of isopropanol into a round bottom flask, deoxygenate the solution with high-purity nitrogen (99.99%) at 35°C for 10min. Then add 25g of acrylamide (AM), 1.25ml of 10% (w / v) ammonium persulfate (APS) and 1.25ml of 10% (v / v) of N,N,N',N'-tetramethyldi Ethylamine (TEMED), after reacting for 90 minutes, the solution was viscous like honey. Dialysis was performed for three days using a dialysis bag (Spectra / por#2, Spectrum Medical Industries, Los Angeles, CA) with a molecular weight cut-off of 12-14 KDa. After dialysis, the polymer solution was freeze-dried to obtain a white solid with a reaction yield of 80%.

Embodiment 2

[0033] Embodiment 2, the preparation of poly N, N-dimethylacrylamide

[0034] Add 220ml of deionized water, 7.6ml of isopropanol and 24ml of N,N-dimethylacrylamide into a round bottom flask, deoxygenate the solution with high-purity nitrogen (99.99%) for 30min, put it in a water bath at 50°C for 20min, and then add 0.625ml 10% (w / v) of ammonium persulfate (APS) and 0.625ml 10% (v / v) of N,N,N',N'-tetramethyldiethylamine (TEMED), placed at 50 ℃ water bath after reaction for 3h. Dialysis was performed for three days using a dialysis bag (Spectra / por#2, Spectrum Medical Industries, Los Angeles, CA) with a molecular weight cut-off of 12-14 KDa. After dialysis, the polymer solution was freeze-dried to obtain a white solid with a reaction yield of 89%.

Embodiment 3

[0035] Embodiment 3, the preparation of mixed polymer solution

[0036] 1. Preparation of sol buffer

[0037] Weigh 19g of urea, add it to a clean 100ml volumetric flask, add 5ml 10×310and 31xxRunning Buffer (Applied Biosystems, USA), shake gently by hand until all urea is dissolved, add deionized water to make up to 50ml.

[0038] 2. Preparation of mixed polymer solution

[0039] Polyacrylamide and poly-N,N-dimethylacrylamide according to the ratio of 1.5:1, add 50ml of sol buffer, and swell overnight. Use a 0.2μm filter to filter and collect the filtrate into a clean volumetric flask. Store at 4°C to prevent urea degradation.

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PUM

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Abstract

The invention discloses mixed polymer solution for capillary electrophoresis and a method for preparing the mixed polymer solution. The method includes mixing polyacrylamide, poly-N, N-dimethyl acrylamide and sol buffer solution with one another to obtain the mixed polymer solution. The mixed polymer solution and the method have the advantages that the mixed polymer solution prepared by the aid ofthe method can have excellent DNA (deoxyribonucleic acid) separating capacity and self-coating capacity in capillary electrophoresis procedures, and the method is simple and can be implemented in laboratories for preparing the mixed polymer solution; the mixed polymer solution which is a product prepared by the aid of the method can have high economic benefits in production application.

Description

technical field [0001] The invention relates to the field of capillary electrophoresis, in particular to a mixed polymer solution for capillary electrophoresis and a preparation method thereof. Background technique [0002] Polyacrylamide is currently the best medium for DNA separation among all polymers. However, polyacrylamide has no self-coating ability, so a coating must be added to the inner wall of the capillary to eliminate the adsorption and electroosmotic flow of DNA on the inner wall of the capillary, so as to effectively separate DNA fragments. In addition, high molecular weight polyacrylamide has a high viscosity and requires high pressure to pour it into the capillary. [0003] Poly N,N-dimethylacrylamide has a strong self-coating ability, which can eliminate the adsorption of DNA and reduce electroosmotic flow. Its viscosity is also much lower than that of polyacrylamide, but its effect of separating DNA fragments is lower than that of polyacrylamide. Acrylam...

Claims

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Application Information

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IPC IPC(8): C08J3/03C08L33/26C08L33/24C12N15/10
CPCC08J3/03C08J2333/26C08J2433/24C12N15/1006C12Q2565/125
Inventor 俞丽娟聂燕钗赵颖姜伯玮荣海博管桦张涛金川
Owner THE FIRST RES INST OF MIN OF PUBLIC SECURITY
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