Stable extracting method for anthocyanin in hongye poplar leaves

Abstract

The invention relates to a stable extracting method for anthocyanin in hongye poplar leaves. The stable extracting method specifically comprises the following steps: 1) taking cleaned and air-dried fresh hongye poplar leaves, removing leaf stalks and leaf veins, cutting the hongye poplar leaves into pieces with diameters smaller than 0.5mm, and refrigerating the hongye poplar leaves at a temperature of 5 DEG C for later use; 2) weighing 1.0g of leaf pieces obtained in the step 1), adding 10 +/-1ml of a hydrochloric acid methanol solution with concentration of 1%, and sealing to prevent evaporation; 3) putting the hongye poplar leaves into a constant temperature incubator of 30-35 DEG C, rocking and extracting for 5-6 hours under a dark condition; 4) filtering after extracting is ended, taking 2ml of an extracting solution, diluting the extracting solution with the hydrochloric acid methanol solution with concentration of 1% and keeping constant volume to 10ml to obtain a to-be-tested solution, sealing, and storing under a dark environment without light at a temperature of 0-4 DEG C for testing. The method can efficiently and sufficiently extract anthocyanin in the leaves under unified regulation conditions, and keeps stability of anthocyanin in the extracting solution; and the anthocyanin is stored for 8 hours for degradation, so that concentration can be controlled within 0.86%.

Description

technical field [0001] The invention belongs to the technical field of extraction of plant effective components, and in particular relates to a method for stably extracting anthocyanins from poplar leaves. Background technique [0002] 'Zhonghongyang', 'Quanhongyang' and 'Jinhongyang' are '2025' poplar varieties with colorful leaves discovered successively since 2000. Obtained the national protection rights for new varieties of forest trees. Professor Huang Dongsen, a poplar breeding expert of the Chinese Academy of Forestry, said: "This tree species fills a gap in poplar breeding in my country." A poplar expert from Oregon, USA was very surprised when he saw this tree species at the Shandong Tai'an Green Expo Lian said: "Rare treasures, unique in the world." The color of poplar leaves is the core indicator of its ornamental quality, and also an important factor affecting its commercial value. The difference in anthocyanin content and proportion is the cause of the leaves o...

AI Technical Summary

Problems solved by technology

In the process of dynamic determination and analysis of anthocyanins in the leaves of Populus red leafe in the growth period by the existing method, due to the change of the measurement time and environment, the experimental error is often large, resulting in long time-consuming, high loss, and reducing the credibility of the results

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