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Culture medium for tissue culture of cibotium barometz spores

A technique for spore group and culture medium, which is applied in the field of spore culture medium of golden retriever spore, can solve the problems of complex seedling breeding, scarce wild resources, long cycle, etc., and achieve nutrient-rich, comprehensive, nutrient-rich, transplanting The effect of high survival rate

Inactive Publication Date: 2018-02-23
傅明尧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the variety of Chinese patent medicines containing dog spines in domestic pharmaceutical companies, the demand for dog spines has increased, leading to crazy digging among the people, and wild resources have become increasingly scarce. In addition, the breeding of artificially planted dog spines is complicated. The cost of stem section propagation is high, the cycle is long, and it is not suitable for promotion; the reproduction coefficient of spore artificial environment is low, it is not suitable for large-scale breeding, and there are few researches on tissue culture

Method used

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Effect test

Embodiment 1

[0016] A culture medium for the tissue culture of chrysospores of Golden Retriever, consisting of the following components and their concentrations: improved MS medium 5g / L, sucrose 20g / L, potato extract 225g / L, melamine alcohol 0.7mg / L , cinnamic acid 0.6g / L, jasmonic acid 0.05mg / L, oxytetracycline 0.18g / L; the improved components in the improved MS medium are: MgSO4 7H2O adjusted to 190mg / L; CaCl2 2H2O adjusted to 210mg / L ; KNO3 is adjusted to 650mg / L, (NH)4NO3 is adjusted to 550mg / L; KH2PO4 is adjusted to 180mg / L; (NH)2HPO4 is increased to 370mg / L.

Embodiment 2

[0018] A kind of golden retriever chrysospore tissue culture medium, which is composed of the following components and the components of the concentration: improved MS medium 4g / L, sucrose 10g / L, potato extract 200g / L, melamine alcohol 0.5mg / L, Cinnamic acid 0.1g / L, jasmonic acid 0.02mg / L, oxytetracycline 0.15g / L; The composition improved in the improved MS medium is: MgSO4 7H2O is adjusted to 180mg / L; CaCl2 2H2O is adjusted to 200mg / L L; KNO3 adjusted to 600mg / L, (NH)4NO3 adjusted to 500mg / L; KH2PO4 adjusted to 170mg / L; increase (NH)2HPO4 350mg / L.

Embodiment 3

[0020] A kind of culture medium of chrysospore golden retriever, which is composed of the following components and the components of the concentration: 5g / L of improved MS medium, 30g / L of sucrose, 250g / L of potato extract, 1mg / L of meliflora, cinnamon Acid 1g / L, jasmonic acid 0.1mg / L, oxytetracycline 0.2g / L; The composition improved in the improved MS medium is: MgSO4.7H2O is adjusted to 200mg / L; CaCl2.2H2O is adjusted to 220mg / L; KNO3 is adjusted to 700mg / L, (NH)4NO3 is adjusted to 600mg / L; KH2PO4 is adjusted to 200mg / L; (NH)2HPO4 is increased to 400mg / L.

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Abstract

The invention discloses a medium for tissue culture of spores of golden retriever dog spores, which is composed of the following components and their concentrations: 4-5g / L of improved MS medium, 10-30g / L of sucrose, and 200-250g of potato extract / L, melissa alcohol 0.5-1mg / L, cinnamic acid 0.1-1g / L, jasmonic acid 0.02-0.1mg / L, oxytetracycline 0.15-0.2g / L. The culture medium of the invention has the advantages of rich and comprehensive nutrition, high spore germination rate, robust tissue cultured seedlings obtained, good rooting effect and high transplanting survival rate, and has good market prospects.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a tissue culture medium for spores of the golden retriever dog. Background technique [0002] Cibotium barometz (L.) J.Sm. is a fern of the clam shell fern family. It is recorded in the "Dictionary of Traditional Chinese Medicine": the golden dog ridge is born on the side of the mountain foot, or in the acidic soil in the shade of the forest. Distributed in the southwest, south, southeast of my country, Henan, Hubei and other places, mainly produced in Sichuan, Fujian, and Zhejiang. In addition, it is also produced in Guangxi, Guangdong, Guizhou, Jiangxi, Hubei and other places. [0003] The rhizome of Golden Retriever Dog’s Ridge, which is dried and depilated, is the traditional Chinese medicine Dog’s Ridge, which was first recorded in "Shen Nong’s Herbal Classic", and has been recorded in all previous editions of "Chinese Pharmacopoeia". , for the treatment of soren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 傅明尧
Owner 傅明尧
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