Donkey-hide gelatin active peptide, composition and preparation method
A technology for active peptides and compositions, applied in the biological field, can solve the problems that the rotational speed of an industrial centrifuge cannot be reached, is difficult to conceal, and is not suitable for industrial production, etc., and achieves the effects of better overall taste, high extraction rate, and improved overall taste.
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[0031]
[0032] In order to eliminate the bitterness and peculiar smell of donkey-hide gelatin active peptide, the preparation method of donkey-hide gelatin active peptide of the present invention comprises the steps:
[0033](1) carrying out enzymolysis treatment to donkey-hide gelatin powder to obtain an enzymolysis solution, and then performing an enzyme-inactivating treatment to obtain an enzymolysis solution after inactivating the enzyme;
[0034] (2) Filter the enzymolysis solution after the deenzyme is eliminated through a plate and frame filter press, and then filter through a ceramic membrane to obtain the active peptide permeate; the plate and frame filter press contains a combined filter aid, and the The combined filter aid is composed of activated carbon, perlite and diatomite in a weight ratio of 6-7:1-2:2-3;
[0035] (3) The active peptide filtrate is concentrated and spray-dried to obtain donkey-hide gelatin active peptide powder; wherein, the spray-drying is ...
Embodiment 1
[0058] The preparation method of donkey-hide gelatin active peptide, comprises the following steps:
[0059] (1) Carrying out enzymolysis treatment of donkey-hide gelatin to obtain an enzymolysis solution, the enzymolysis treatment includes:
[0060] A, dry donkey-hide gelatin is carried out ultrafine pulverization, cross 400 mesh sieves, obtain donkey-hide gelatin powder; Add donkey-hide gelatin powder to the water of 4 times of weight of donkey-hide gelatin powder, mix, adjust to pH8 with sodium hydroxide solution, soak 20h; Stir, centrifuge, and take the supernatant;
[0061] B. The supernatant is adjusted to a pH value of 5 with acetic acid solution, the protein is precipitated, mechanically stirred, centrifuged, and the solid part is taken;
[0062] C. Add water to the solid part, heat at 90°C for 20 minutes, and then homogenize to obtain a suspension;
[0063] D. Adjust the pH value of the suspension to 8, add an enzyme preparation for enzymolysis to obtain an enzymoly...
Embodiment 2
[0068] The preparation method of donkey-hide gelatin active peptide, comprises the following steps:
[0069] (1) Carrying out enzymolysis treatment of donkey-hide gelatin to obtain an enzymolysis solution, the enzymolysis treatment includes:
[0070] A, dry donkey-hide gelatin is carried out ultrafine pulverization, cross 400 mesh sieves, obtain donkey-hide gelatin powder; Add donkey-hide gelatin powder to the water of 4 times of weight of donkey-hide gelatin powder, mix, adjust to pH9 with sodium hydroxide solution, soak 24h; Stir, centrifuge, and take the supernatant;
[0071] B. The supernatant is adjusted to a pH value of 4 with a citric acid solution, the protein is precipitated, mechanically stirred, centrifuged, and the solid part is taken;
[0072] C. Add water to the solid part, heat at 80°C for 30min, and then homogenize to obtain a suspension;
[0073] D. Adjust the pH value of the suspension to 6, add an enzyme preparation for enzymolysis to obtain an enzymolysis s...
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