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Bone marrow stem cell protection solution and preparation method thereof

A technology for bone marrow stem cells and protective liquid, which is applied in the field of bone marrow stem cell protective liquid and its preparation, can solve the problems of harsh use conditions, difficult clinical application, low cell survival rate, etc., and achieves the effect of stable performance and expanding the scope of clinical application.

Inactive Publication Date: 2018-02-09
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bone marrow stem cell protection solutions disclosed in the prior art generally have problems such as low cell survival rate, poor safety, and relatively harsh conditions of use, and there are certain difficulties in clinical application.

Method used

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  • Bone marrow stem cell protection solution and preparation method thereof
  • Bone marrow stem cell protection solution and preparation method thereof
  • Bone marrow stem cell protection solution and preparation method thereof

Examples

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Effect test

Embodiment 1

[0034] A bone marrow stem cell protection solution, the protection solution is mainly formed by dissolving a hypoxia protective agent, heparin sodium and gentamycin with a DMEM / F12 medium aqueous solution, and each mL of the DMEM / F12 medium aqueous solution dissolves the Hypoxia protective agent 8 mg, the heparin sodium 0.02 U, the gentamicin 100 U, the concentration of the DMEM / F12 medium aqueous solution is 33.7 mg / mL.

Embodiment 2

[0036] A bone marrow stem cell protection solution, the protection solution is mainly formed by dissolving a hypoxia protective agent, heparin sodium and gentamycin with a DMEM / F12 medium aqueous solution, and each mL of the DMEM / F12 medium aqueous solution dissolves the Hypoxia protective agent 5mg, the heparin sodium 0.01U, the gentamicin 50U, the concentration of the DMEM / F12 medium aqueous solution is 30mg / mL.

Embodiment 3

[0038] A bone marrow stem cell protection solution, the protection solution is mainly formed by dissolving a hypoxia protective agent, heparin sodium and gentamycin with a DMEM / F12 medium aqueous solution, and each mL of the DMEM / F12 medium aqueous solution dissolves the Hypoxia protective agent 10 mg, the heparin sodium 0.05 U, the gentamicin 200 U, the concentration of the DMEM / F12 medium aqueous solution is 40 mg / mL.

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Abstract

The present invention provides a bone marrow stem cell protection solution, which is mainly prepared by dissolving a hypoxic protection agent, heparin sodium and an aminoglycoside antibiotic in a DMEM / F12 culture medium aqueous solution, wherein per mL of the DMEM / F12 culture medium aqueous solution can respectively dissolve 5-10 mg of the hypoxic protection agent, 0.01-0.05 U of the heparin sodium and 50-200 U of the aminoglycoside antibiotic, and the concentration of the DMEM / F12 culture medium aqueous solution is 30-40 mg / ml. According to the present invention, the bone marrow stem cell protection solution has advantages of stable performance, safety and no toxicity, can effectively protect bone marrow stem cells, can make bone marrow stem cells be preserved for a long time and can maintain the cell activity in vitro, can maintain the original multidirectional differentiation ability, and can expand the clinical application of bone marrow stem cells, wherein the bone marrow stem cells can be transported to most domestic cities and neighboring countries by using the existing transportation tools.

Description

technical field [0001] The invention belongs to the technical field of bone marrow stem cell preservation, and in particular relates to a bone marrow stem cell protection solution and a preparation method thereof. Background technique [0002] Bone marrow stem cells are a kind of pluripotent cells with self-replication ability. They have the characteristics of self-replication, self-renewal, multi-directional differentiation potential, hematopoietic support, and immune regulation. They have broad clinical applications in hematopoietic reconstruction, tissue repair, and immunotherapy. Prospect; and bone marrow stem cells still have multi-directional differentiation potential after continuous subculture and cryopreservation recovery in vitro, and can be used as ideal seed cells for the repair of tissue and organ damage caused by aging and disease, and are the most promising cell therapy technology One of the source cells. Therefore, it is particularly important to study an ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 李霞云曹毓琳白志惠刘世红卢承前
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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